Mobilisation and dysfunction of haematopoietic stem/progenitor cells after Listonella anguillarum infection in ayu, Plecoglossus altivelis.
Mobilisation and dysfunction of haematopoietic stem/progenitor cells after Listonella anguillarum infection in ayu, Plecoglossus altivelis.
复制标题
香鱼、香鱼利斯顿氏菌感染后造血干/祖细胞的动员和功能障碍
DOI：
10.1038/srep28082
复制
发表时间：
2016-06-16
影响因子：
4.6
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Lu XJ;Chen Q;Rong YJ;Chen J
造血干/祖细胞（HSPC）可以动员到血液中，并在应激后产生免疫细胞谱系。然而，HSPCs感染后在硬骨鱼类中的稳态和功能还不太清楚。在这里，我们报告说，Listonella anguillarum感染增强HSPC动员，并减少其分化成髓细胞的香鱼（Plecoglossus altivelis），在东亚的水产养殖硬骨鱼。我们建立了一个集落形成单位培养（CFU-C）测定，以测量HSPCs使用条件培养基从外周血单个核细胞刺激与植物血凝素。香鱼感染L后，头肾CFU-Cs数量减少，血液和脾脏CFU-Cs数量增加。鳗属L.后HSPC动员去甲肾上腺素介导了鳗的感染。此外，香鱼HSPCs经L.鳗脂多糖（LPS）表现出髓样分化缺陷，不能再拯救L.鳗感染的香鱼在L.鳗感染或其LPS处理。这些结果揭示了硬骨鱼HSPC调控与病原体感染之间的联系。
Haematopoietic stem/progenitor cells (HSPCs) can mobilise into blood and produce immune cell lineages following stress. However, the homeostasis and function of HSPCs after infection in teleosts are less well known. Here, we report that Listonella anguillarum infection enhances HSPC mobilisation and reduces their differentiation into myeloid cells in ayu (Plecoglossus altivelis), an aquacultured teleost in East Asia. We established a colony-forming unit culture (CFU-C) assay to measure HSPCs using conditioned medium from peripheral blood mononuclear cells stimulated with phytohaemagglutinin. The number of CFU-Cs decreased in the head kidney and increased in the blood and spleen of ayu infected with L. anguillarum. HSPC mobilisation after L. anguillarum infection was mediated by norepinephrine. Furthermore, HSPCs from ayu treated with L. anguillarum lipopolysaccharide (LPS) showed defective myeloid differentiation and could no longer rescue L. anguillarum-infected ayu. HSPC expansion was suppressed after L. anguillarum infection or its LPS treatment in vitro. These results reveal a link between HSPC regulation and pathogen infection in teleosts.