Uptake of lithium carmine by sinusoidal endothelial and Kupffer cells of the rat liver: new insights into the classical vital staining and the reticulo-endothelial system
Uptake of lithium carmine by sinusoidal endothelial and Kupffer cells of the rat liver: new insights into the classical vital staining and the reticulo-endothelial system
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DOI：
10.1007/s004410051069
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发表时间：
1998-05-01
影响因子：
3.6
通讯作者：
Wake, K
中科院分区：
文献类型：
--
作者：
Kawai, Y;Smedsrod, B;Wake, K
采用原始的胭脂红锂活体染色法对大鼠肝脏窦细胞进行了体内和体外研究，为网状内皮系统概念的发展做出了重要贡献。免疫组织化学和电镜检查显示，合并染料的细胞为窦状内皮细胞、库普弗细胞和单核细胞。内皮细胞比库普弗细胞吸收了更多的染料，并在窦腔内大量凸起。电镜显示，小颗粒的胭脂红锂与内皮细胞的包被囊泡和Kupffer细胞的皱褶膜有关。在内皮细胞中，这些颗粒以不同浓度存在于液泡结构中，并在溶酶体中凝聚，形成大的胭脂红锂块聚集体。这些块状物显示出晶体结构，其中单个颗粒的宽度可达30纳米，长度可达50纳米。少量含有丰富染料的内皮细胞发生变性，部分被Kupffer细胞占据。胭脂红锂给药大鼠肝内皮细胞内吞荧光标记胶原。从正常大鼠肝脏分离的内皮细胞，当用胭脂红锂培养时，不沾染任何染料，甲醛处理的白蛋白的内吞作用被剂量依赖性地抑制。我们得出结论，在肝脏中，内皮细胞，而不是Kupffer细胞，主要吸收锂胭脂红。此外，我们提出了一个基于其重要染色能力的广义细胞系统的存在。
Sinusoidal cells in the rat liver were studied in vivo and in vitro using the original vital staining with lithium carmine, which has contributed much to the development of the concept of the reticulo-endothelial system. Immunohistochemical and electron-microscopic studies revealed that the dye-incorporating cells were sinusoidal endothelial cells, Kupffer cells, and monocytes. The endothelial cells took up much more dye than did the Kupffer cells and bulged largely into the sinusoidal lumen. Electron microscopy revealed that small particles of Lithium carmine were associated with coated vesicles of endothelial cells and ruffled membranes of Kupffer cells. In the endothelial cells, these particles were present in various concentrations within vacuolated structures and condensed in the lysosomes forming large aggregates of lithium carmine lumps. These lumps showed crystalline structures, within which the size of the individual particle was up to 30 nm in width and 50 nm in length. A few endothelial cells containing abundant dye underwent degeneration, and some were taken up by Kupffer cells. Liver endothelial cells isolated from lithium carmine-administered rats endocytosed fluorescence-labeled collagen. Isolated endothelial cells from normal rat liver, when cultured with lithium carmine, did not take up any dye, and their endocytosis of formaldehyde-treated albumin was inhibited dose-dependently. We conclude that in the liver, endothelial cells, but not Kupffer cells, predominantly take up lithium carmine. Furthermore, we propose the existence of a generalized cell system based on its vital staining capacity.