EHRLICHIA-CHAFFEENSIS, A NEW SPECIES ASSOCIATED WITH HUMAN EHRLICHIOSIS
EHRLICHIA-CHAFFEENSIS, A NEW SPECIES ASSOCIATED WITH HUMAN EHRLICHIOSIS
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DOI：
10.1128/jcm.29.12.2838-2842.1991
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发表时间：
1991-12-01
影响因子：
9.4
通讯作者：
WILSON, KH
中科院分区：
文献类型：
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作者：
ANDERSON, BE;DAWSON, JE;WILSON, KH
用聚合酶链反应扩增了两名埃立克体病患者血液样品中的细菌16S rRNA基因和其中一名患者的分离株。然后对扩增物进行克隆和测序。还确定了犬埃里希体（两株）、E. equi、E.嗜吞噬细胞E. sennetsu（两种菌株）。这些序列沿着已发表的E. Ristiopathy，进行了比较。所有三种来源的人埃立克体病病原体的16S rRNA基因序列相同。序列比较表明，人埃立克体病病原体是一个与E.犬埃立克体（98.2%）与其它埃立克体属亲缘关系较远。我们建议将该种命名为查菲埃里希体（Ehrlichia chaffeensis sp. nov.）以阿肯色州菌株为模式菌株。
The bacterial 16S rRNA genes from blood samples of two patients with human ehrlichiosis and from an isolate recovered from one of the patients were amplified by using the polymerase chain reaction. The amplimers were then cloned and sequenced. The 16S rRNA gene sequence was also determined for Ehrlichia canis (two strains), E. equi, E. phagocytophila (two strains), and E. sennetsu (two strains). These sequences, along with a previously published 16S rRNA gene sequence of E. risticii, were compared. The 16S rRNA gene sequences were identical for all three sources of the human ehrlichiosis agent. The sequence comparisons indicate that the human ehrlichiosis agent is a new species most closely related to E. canis (98.2%) and more distantly related to other Ehrlichia spp. We propose that this species be named Ehrlichia chaffeensis sp. nov., with the Arkansas strain as the type strain.