Regulation of enzymes involved in the biosynthesis of the sesquiterpene antibiotic pentalenolactone in Streptomyces arenae.
Regulation of enzymes involved in the biosynthesis of the sesquiterpene antibiotic pentalenolactone in Streptomyces arenae.
复制标题
链霉菌中倍半萜抗生素五烯内酯生物合成所涉及的酶的调节。
DOI：
10.7164/antibiotics.39.266
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
D. Mecke
中科院分区：
文献类型：
--
作者：
K. Maurer;D. Mecke
在产生菌TU 469中，倍半萜类抗生素戊烯内酯的产生受法尼基焦磷酸环化酶的活性控制。与这种酶的活性相反，到目前为止测试的甲伐酮途径的所有其他酶的比活性被证明不是限速的。检测了戊烯内酯途径的几种代谢产物对HMG-CoA还原酶和法尼基焦磷酸环化酶活性的抑制作用。低浓度的戊烯内酯及其衍生物对环化酶的活性有抑制作用，提示戊烯内酯途径的起始酶最终被抑制。戊二烯或任何戊烯内酯衍生物不抑制HMG-CoA还原酶的活性。根据这些结果，对第一种戊烯内酯合成专一性酶的最终产物的抑制似乎是参与调节戊烯内酯生物合成的一种机制。
The production of the sesquiterpenoid antibiotic pentalenolactone in the producer strain Streptomyces arenae TU 469 is controlled by the activity of the enzyme farnesylpyrophosphate cyclase. In contrast to the activity of this enzyme, the specific activities of all other enzymes of the mevalonoid pathway tested so far, proved to be not rate-limiting. Several metabolites of the pentalenolactone pathway were tested for inhibitory effects on the activity of the HMG-CoA reductase and farnesylpyrophosphate cyclase. The activity of the cyclase was inhibited by low concentrations of pentalenolactone and its derivatives, thus suggesting an end product inhibition of the starting enzyme of the pentalenolactone pathway. The activity of HMG-CoA reductase was not inhibited by pentalenene or any pentalenolactone-derivatives. According to these results, an end-product inhibition of the first enzyme which is specific for pentalenolactone synthesis seems to be a mechanism involved in the regulation of pentalenolactone biosynthesis.