Marker assisted selection to improve HMW-glutenins in wheat
Marker assisted selection to improve HMW-glutenins in wheat
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DOI：
10.1023/a:1017534203520
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发表时间：
2001-01-01
期刊：
影响因子：
1.9
通讯作者：
Jouve, N
中科院分区：
文献类型：
--
作者：
de Bustos, A;Rubio, P;Jouve, N
本研究利用PCR技术扩增小麦高分子量麦谷蛋白基因特异等位基因的全编码序列。利用Glu-A1基因x1、x2 *、x5、y10、y12序列间的微小差异，设计了一套Glu-A1和Glu-D1基因的AS-PCR分子标记。这些标记被应用于辅助选择回交后代，以提高西班牙小麦的谷蛋白品质。利用其它多态性系统（AFLP）恢复轮回亲本的遗传背景也有助于选择。
The present work presents the application of new markers based on the PCR technology to amplify the complete coding sequence of the specific alleles of the high molecular weight (HMW) glutenin genes. A set of AS-PCR molecular markers of the Glu-A1 and Glu-D1 genes was designed, making use of the minor differences between the sequences of the x1, xNull and x2* of Glu-A1, x2, x5, y10 and y12 of Glu-D1. These markers were applied to assist selection in backcross progenies to improve the glutenin quality in Spanish wheat. The selection was also assisted using other polymorphic systems (AFLPs) in recovering the genetic background of the recurrent parent.