A Recombinant Chlamydia trachomatis MOMP Vaccine Elicits Cross-serogroup Protection in Mice Against Vaginal Shedding and Infertility.
A Recombinant Chlamydia trachomatis MOMP Vaccine Elicits Cross-serogroup Protection in Mice Against Vaginal Shedding and Infertility.
复制标题
重组沙眼衣原体 MOMP 疫苗可对小鼠产生跨血清群保护，防止阴道脱落和不孕。
DOI：
10.1093/infdis/jiz438
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
delaMaza,LuisM
中科院分区：
文献类型：
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作者：
Tifrea,DeliaF;Pal,Sukumar;delaMaza,LuisM
研究背景沙眼衣原体（Chlamydia trachomatisis）是世界范围内最常见的性传播病原体.在这里，我们确定了aC的能力。方法用沙眼衣原体重组主要外膜蛋白（rMOMP）疫苗免疫雌性C3 H/HeN小鼠，经粘膜和全身途径接种沙眼衣原体，观察其免疫保护作用。沙眼衣原体血清型D（UW-3/Cx）rMOMP，并用血清型D（UW-3/Cx）、D（UCI-96/Cx）、E（IOL-43）或F（N.I.1）在卵巢囊中攻击。CpG-1826和Montanide伊萨720被用作adjuvant. ResultsThe免疫接种后的反应更强大的最密切相关的血清型。生殖器激发后（根据阴道培养物阳性的小鼠数量、阳性培养物数量、回收的包涵体形成单位数量和阳性培养物天数确定），用C.同一复合物的沙眼衣原体血清型受到保护，但用不同亚复合物的血清型F（N.I.1）攻击的沙眼衣原体血清型则没有受到保护。将雌性小鼠与雄性小鼠关在一起。根据生育率，胚胎数量和输卵管积水的形成，免疫小鼠保护免受挑战血清型D（UW-3/Cx），D（UCI-96/Cx），和E（IOL-43），但不是F（N.I.1）。沙眼类型
BackgroundChlamydia trachomatisis the most common sexually transmitted bacterial pathogen worldwide. Here, we determined the ability of aC. trachomatisrecombinant major outer membrane protein (rMOMP) vaccine to elicit cross-serogroup protection.MethodsFemale C3H/HeN mice were vaccinated by mucosal and systemic routes withC. trachomatisserovar D (UW-3/Cx) rMOMP and challenged in the ovarian bursa with serovars D (UW-3/Cx), D (UCI-96/Cx), E (IOL-43), or F (N.I.1). CpG-1826 and Montanide ISA 720 were used as adjuvants.ResultsImmune responses following vaccination were more robust against the most closely related serovars. Following a genital challenge (as determined by number of mice with positive vaginal cultures, number of positive cultures, number of inclusion forming units recovered, and number of days with positive cultures) mice challenged withC. trachomatisserovars of the same complex were protected but not those challenged with serovar F (N.I.1) from a different subcomplex. Females were caged with male mice. Based on fertility rates, number of embryos, and hydrosalpinx formation, vaccinated mice were protected against challenges with serovars D (UW-3/Cx), D (UCI-96/Cx), and E (IOL-43) but not F (N.I.1).ConclusionsThis is the first subunit vaccine shown to protect mice against infection, pathology, and infertility caused by differentC. trachomatisserovars.