Myricetin protects against H2O2-induced oxidative damage and apoptosis in bovine mammary epithelial cells
Myricetin protects against H2O2-induced oxidative damage and apoptosis in bovine mammary epithelial cells
复制标题
杨梅素通过促进 AMPK/NRF2 信号传导来防止 H2O2 诱导的牛乳腺上皮细胞氧化损伤和凋亡
DOI：
10.1002/jcp.30035
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发表时间：
2020-09-03
影响因子：
5.6
通讯作者：
Fu, Shoupeng
中科院分区：
文献类型：
--
作者：
Kan, Xingchi;Liu, Juxiong;Fu, Shoupeng
High-producing dairy cows are prone to oxidative stress due to their high secretion and strong metabolism, and excessive oxidative stress may cause the apoptosis of bovine mammary epithelial cells (bMECs). Myricetin (Myr) has been shown to have a wide range of pharmaceutical activities. The aim of this study was to evaluate the effect of Myr on hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in bMECs and to clarify the underlying mechanism. bMECs were pretreated with or without Myr and then stimulated with H2O2. The results showed that Myr significantly increased the total antioxidant capacity and superoxide dismutase levels and decreased the malondialdehyde (MDA) and reactive oxygen species (ROS) levels in a model of oxidative stress induced by H(2)O(2)in bMECs. Mechanistic studies found that Myr inhibited H2O2-induced oxidative stress in bMECs through the adenosine monophosphate-activated protein kinase/nuclear factor erythroid-2 related factor 2 (AMPK/NRF2) signaling pathway. Additional research found that Myr could also inhibit H2O2-induced apoptosis in bMECs through NRF2. These data suggest that Myr effectively alleviated oxidative stress and apoptosis in H2O2-induced bMECs through the activation of the AMPK/NRF2 signaling pathway.
