The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins.
The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins.
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DOI：
10.1007/s10969-010-9094-7
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发表时间：
2010-09
期刊：
影响因子：
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通讯作者：
Hendrickson, Wayne A
中科院分区：
文献类型：
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作者：
Love, James;Mancia, Filippo;Shapiro, Lawrence;Punta, Marco;Rost, Burkhard;Girvin, Mark;Wang, Da-Neng;Zhou, Ming;Hunt, John F;Szyperski, Thomas;Gouaux, Eric;MacKinnon, Roderick;McDermott, Ann;Honig, Barry;Inouye, Masayori;Montelione, Gaetano;Hendrickson, Wayne A
纽约膜蛋白结构联盟（NYCOMPS）的成立是为了利用结构基因组学方法加速获取膜蛋白的结构信息。NY-COMPS包括一个生物信息学小组，一个操作高通量克隆和筛选管道的集中设施，一组相关的湿实验室，通过x射线晶体学和核磁共振进行高水平蛋白质生产和结构测定，以及一组专注于方法开发的研究人员。在运营的前三年，NYCOMPS管道迄今已为8045个目标蛋白生产和筛选了7250个表达构建。大约有600个已验证的靶点被放大到结构研究所需的水平，到目前为止产生了24个膜蛋白晶体。在这里，我们描述了NYCOMPS的整体结构，并提供了高吞吐量管道的详细信息。
The New York Consortium on Membrane Protein Structure (NYCOMPS) was formed to accelerate the acquisition of structural information on membrane proteins by applying a structural genomics approach. NY-COMPS comprises a bioinformatics group, a centralized facility operating a high-throughput cloning and screening pipeline, a set of associated wet labs that perform high-level protein production and structure determination by x-ray crystallography and NMR, and a set of investigators focused on methods development. In the first three years of operation, the NYCOMPS pipeline has so far produced and screened 7,250 expression constructs for 8,045 target proteins. Approximately 600 of these verified targets were scaled up to levels required for structural studies, so far yielding 24 membrane protein crystals. Here we describe the overall structure of NYCOMPS and provide details on the high-throughput pipeline.