Leptin and TNF-alpha promoter methylation levels measured by MSP could predict the response to a low-calorie diet
Leptin and TNF-alpha promoter methylation levels measured by MSP could predict the response to a low-calorie diet
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DOI：
10.1007/s13105-011-0084-4
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发表时间：
2011-09-01
影响因子：
3.4
通讯作者：
Alfredo Martinez, J.
中科院分区：
文献类型：
--
作者：
Cordero, Paul;Campion, Javier;Alfredo Martinez, J.
Obesity-associated adipose tissue enlargement is characterized by an enhanced proinflammatory status and an elevated secretion of adipokines such as leptin and cytokines such as tumor necrosis factor (TNF)-alpha. Among the different mechanisms that could underlie the interindividual differences in obesity, epigenetic regulation of gene expression has emerged as a potentially important determinant. Therefore, 27 obese women (age, 32-50 years; baseline body mass index, 34.4 +/- 4.2 kg/m(2)) were prescribed an 8-week low-calorie diet, and epigenetic marks were assessed. Baseline and endpoint anthropometric parameters were measured, and blood samples were drawn. Genomic DNA and RNA from adipose tissue biopsies were isolated before and after the dietary intervention. Leptin and TNF-alpha promoter methylation were measured by MSP after bisulfite treatment, and gene expression was also analyzed. Obese women with a successful weight loss (>= 5% of initial body weight, n=21) improved the lipid profile and fat mass percentage (-12%, p
