Age-related changes in endothelium-dependent relaxation in aorta from genetically diabetic WBN/Kob rats.
Age-related changes in endothelium-dependent relaxation in aorta from genetically diabetic WBN/Kob rats.
复制标题
遗传性糖尿病 WBN/Kob 大鼠主动脉内皮依赖性松弛的年龄相关变化。
DOI：
10.1152/ajpheart.1992.262.4.h1104
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Y. Kasuya
中科院分区：
文献类型：
--
作者：
N. Miyata;K. Tsuchida;S. Okuyama;S. Otomo;K. Kamata;Y. Kasuya
用Wistar大鼠(对照组)和WBN/KOB(遗传性糖尿病)大鼠，观察衰老和高血糖对内皮依赖性和非内皮依赖性药物的主动脉舒张性的影响。老年(90~92wk)WBN/KOB大鼠血清葡萄糖浓度显著升高，而青年(13~15wk)WBN/KOB大鼠血清葡萄糖浓度未见明显升高。对照组和WBN/KOB大鼠对乙酰胆碱的内皮依赖性松弛随着年龄的增长而降低。与年龄匹配的对照血管相比，青年(非糖尿病)和老年(糖尿病)WBN/KOB大鼠的主动脉条对乙酰胆碱的松弛作用均显著减弱。老年WBN/KOB大鼠和老年WBN/KOB大鼠主动脉条硝普钠的量效曲线较青年大鼠分别左移。然而，硝普钠的最大松弛反应在所有被研究的血管中没有差异。硝普钠对WBN/KOB幼年大鼠和老年WBN/KOB大鼠主动脉条的舒缩作用分别与年龄匹配的对照组大鼠相似。心钠素引起的血管舒缩作用在所有研究的血管中没有差异。在遗传性糖尿病大鼠中，内皮功能的改变发生在血清葡萄糖水平升高之前。因此，内皮依赖性松弛功能受损可能在糖尿病血管并发症的高发生率中起重要作用。
Experiments were designed to investigate the effects of aging and hyperglycemia on relaxation of the aorta for both endothelium-dependent and -independent agents in Wistar (control) and WBN/Kob (genetically diabetic) rats. The concentration of glucose in serum was elevated significantly in aged (90-92 wk) but not young (13-15 wk) WBN/Kob rats. Endothelium-dependent relaxations of both control and WBN/Kob rats to acetylcholine were reduced by aging. The relaxations induced by acetylcholine in aortic strips were significantly attenuated in both young (nondiabetic) and aged (diabetic) WBN/Kob rats, compared with those from age-matched control vessels, respectively. The concentration-response curves for sodium nitroprusside in aortic strips from both aged control and aged WBN/Kob rats were shifted to the left when compared with those from young rats, respectively. However, the maximal relaxation responses to sodium nitroprusside showed no difference among all vessels studied. The relaxations induced by sodium nitroprusside in aortic strips from both young and aged WBN/Kob rats were similar to those from age-matched control rats, respectively. The relaxations induced by atrial natriuretic peptide showed no difference among all vessels studied. In genetically diabetic rats, functional changes in endothelium occurred before elevation of the levels of glucose in the serum. Thus impaired endothelium-dependent relaxation may play an important role in the high incidence of vascular complications in diabetes mellitus.