Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research.
Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research.
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DOI：
10.3390/ijms161023143
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发表时间：
2015-09-24
影响因子：
5.6
通讯作者：
Fujii H
中科院分区：
文献类型：
--
作者：
Fujita T;Fujii H
工程化的DNA结合分子如转录激活因子样效应物（TAL或TALE）蛋白和成簇的规则间隔短回文重复序列（CRISPR）和CRISPR相关蛋白（Cas）（CRISPR/Cas）系统已广泛用于各种类型和物种的细胞中的基因组编辑。这些工程化DNA结合分子的序列特异性DNA结合活性也可用于其他目的，如转录激活、转录抑制、染色质修饰、基因组区域的可视化和以基因座特异性方式分离染色质。在这篇综述中，我们描述了这些工程化DNA结合分子在基因组编辑以外的生物学目的中的应用。
Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species. The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner. In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing.