Ultrafast pH-jump two-dimensional infrared spectroscopy
Ultrafast pH-jump two-dimensional infrared spectroscopy
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DOI：
10.1364/ol.44.004937
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发表时间：
2019-10-15
期刊：
影响因子：
3.6
通讯作者：
Baiz, Carlos R.
中科院分区：
文献类型：
--
作者：
Flanagan, Jennifer C.;Baiz, Carlos R.
我们提出了一种 pH 跳跃二维红外 (2D IR) 光谱仪，用于探测从纳秒到毫秒的 pH 依赖性构象变化。该设计将纳秒 355 nm 光源集成到基于脉冲整形器的 2D IR 光谱仪中，以触发笼中质子的解离，然后使用飞秒 2D IR 光谱仪探测后续构象变化。我们观察到末端胺质子化引起的二甘氨酸酰胺 I 模式（C=O 拉伸）的蓝移。该方法将超快 2D IR 的键特异性结构敏感性与触发的构象动力学相结合，为多尺度生物分子转化（例如蛋白质折叠）提供结构途径。 (C) 2019 美国光学学会
We present a pH-jump two-dimensional infrared (2D IR) spectrometer to probe pH-dependent conformational changes from nanoseconds to milliseconds. The design incorporates a nanosecond 355 nm source into a pulse-shaper-based 2D IR spectrometer to trigger dissociation of a caged proton prior to probing subsequent conformational changes with femtosecond 2D IR spectroscopy. We observe a blue shift in the amide I mode (C=O stretch) of diglycine induced by protonation of the terminal amine. This method combines the bond-specific structural sensitivity of ultrafast 2D IR with triggered conformational dynamics, providing structural access to multiscale biomolecular transformations such as protein folding. (C) 2019 Optical Society of America