Evaluation of enzymes involved in proteoglycan degradation in the wall of abdominal aortic aneurysms
Evaluation of enzymes involved in proteoglycan degradation in the wall of abdominal aortic aneurysms
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DOI：
10.1159/000089790
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发表时间：
2006-01-01
影响因子：
1.7
通讯作者：
Gacko, M
中科院分区：
文献类型：
--
作者：
Kowalewski, R;Sobolewski, K;Gacko, M
腹主动脉瘤（AAA）壁是动脉重构的一个极端例子，弹性蛋白、胶原蛋白和蛋白多糖代谢紊乱。本研究的目的是评估参与降解AAA壁中糖胺聚糖链和蛋白聚糖核心蛋白的酶。研究材料包括10个腹主动脉的壁样，5个来自器官供体的正常腹主动脉碎片作为对照。用比色法测定内糖苷酶、外糖苷酶和磺化酶的活性。为了评估基质金属蛋白酶（MMPs），进行了Western blot和酶谱分析。内糖苷酶降解硫酸软骨素4的活性在AAA壁较低。降解硫酸肝素和硫酸皮肤素的内糖苷酶、芳基磺化酶B以及所有被评估的外糖苷酶在AAA壁显示出较高的活性。此外，AAA壁MMP1、MMP2和MMP9的表达也有所增加。酶谱图显示，与正常主动脉壁相比，AAA壁中pro-MMP2活性降低，pro-MMP9存在。糖胺聚糖降解酶和MMPs活性的广泛变化可能影响细胞外基质网络的组织，并导致先前证明的AAA壁蛋白聚糖和糖胺聚糖含量的变化。
The abdominal aortic aneurysm (AAA) wall represents an extreme example of arterial remodeling with disturbed elastin, collagen and proteoglycan metabolism. The aim of this study was to evaluate enzymes involved in the degradation of glycosaminoglycan chains and core proteins of proteoglycans in the AAA wall. The study material consisted of wall samples from 10 AAA. Fragments of 5 normal abdominal aortas from organ donors were used as a control. The activity of endoglycosidases, exoglycosidases and sulfatases was measured using colorimetric methods. To assess matrix metalloproteinases ( MMPs), Western blot and zymography were performed. The activity of endoglycosidase degrading chondroitin-4-sulfate was lower in the AAA wall. Endoglycosidase degrading heparan sulfate and dermatan sulfate, arylosulfatase B, as well as all the exoglycosidases assessed demonstrated higher activities in the AAA wall. Furthermore, increased expression of MMP1, MMP2 and MMP9 was also shown in the AAA wall. Zymography revealed decreased activity of pro-MMP2 and presence of pro-MMP9 in the AAA wall compared to the wall of normal aorta. Extensive changes in the activity of glycosaminoglycan-degrading enzymes and MMPs may influence the organization of the extracellular matrix network and lead to previously demonstrated changes in the proteoglycan and glycosaminoglycan content in the AAA wall.