The Effect of Sleep Deprivation on Cardiac Function and Tolerance to Ischemia-Reperfusion Injury in Male Rats.
The Effect of Sleep Deprivation on Cardiac Function and Tolerance to Ischemia-Reperfusion Injury in Male Rats.
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DOI：
10.5935/abc.20150137
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发表时间：
2016-01
影响因子：
2.6
通讯作者：
Ghasemi A
中科院分区：
文献类型：
--
作者：
Jeddi S;Asl AN;Asgari A;Ghasemi A
睡眠剥夺（SD）与心血管疾病风险增加密切相关。目的：观察丹参对雄性大鼠基础血流动力学功能及心肌缺血再灌注损伤耐受性的影响。SD通过使用花盆法诱导4天。采用Langendorff装置对离体心脏进行灌注，分别在基础状态和缺血再灌注后测定以下参数：左室发展压（LVDP）、心率（HR）和左室压力最大上升和下降速率（±dp/dt）。IR后测定心肌NO_x水平、心肌梗死面积、冠脉流量、心肌肌酸激酶同工酶（CK-MB）和乳酸脱氢酶（LDH），并于研究开始和结束时测定收缩压（SBP）。与对照组相比，SD组LVDP（19%）、+dp/dt（18%）和-dp/dt（21%）的基线水平显著降低（p < 0.05），HR（32%）显著升高。缺血后，SD组心脏HR明显增加，血流动力学功能恢复较对照组慢。SD组缺血再灌注后心脏NOx水平、冠脉流量、心肌梗死面积、心肌CK-MB、LDH均明显增加，且4 d后SD大鼠收缩压明显升高。SD大鼠心脏基础心功能较低，对IR损伤的耐受性较差，这可能与IR后NO产生增加有关。
Sleep deprivation (SD) is strongly associated with elevated risk for cardiovascular disease. To determine the effect of SD on basal hemodynamic functions and tolerance to myocardial ischemia-reperfusion (IR) injury in male rats. SD was induced by using the flowerpot method for 4 days. Isolated hearts were perfused with Langendorff setup, and the following parameters were measured at baseline and after IR: left ventricular developed pressure (LVDP); heart rate (HR); and the maximum rate of increase and decrease of left ventricular pressure (±dp/dt). Heart NOx level, infarct size and coronary flow CK-MB and LDH were measured after IR. Systolic blood pressure (SBP) was measured at start and end of study. In the SD group, the baseline levels of LVDP (19%), +dp/dt (18%), and -dp/dt (21%) were significantly (p < 0.05) lower, and HR (32%) was significantly higher compared to the controls. After ischemia, hearts from SD group displayed a significant increase in HR together with a low hemodynamic function recovery compared to the controls. In the SD group, NOx level in heart, coronary flow CK-MB and LDH and infarct size significantly increased after IR; also SD rats had higher SBP after 4 days. Hearts from SD rats had lower basal cardiac function and less tolerance to IR injury, which may be linked to an increase in NO production following IR.