Delta is a ventral to dorsal signal complementary to Serrate, another Notch ligand in Drosophila wing formation
Delta is a ventral to dorsal signal complementary to Serrate, another Notch ligand in Drosophila wing formation
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DOI：
10.1101/gad.10.4.421
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发表时间：
1996-02-15
影响因子：
10.5
通讯作者：
Jan, YN
中科院分区：
文献类型：
--
作者：
Doherty, D;Feger, G;Jan, YN
果蝇翅缘的形成需要Notch受体，在背侧隔室中需要其配体之一Serrate。我们提供的证据表明，三角洲，其他已知的配体的缺口，也是必不可少的这一过程。三角洲是必需的腹侧细胞在背/腹室边界，其表达是特别升高的二龄翅盘在翅缘形成。此外，异位Delta表达诱导无翅、退化和切割，并导致成体翅组织在背侧隔室中生长。这种效应是由Notch介导的，因为Notch活性的丧失抑制了Delta诱导的异位翅膀生长。而Notch或截短的活化Notch的异位表达诱导背侧和腹侧隔室中的切割，异位Delta表达仅诱导背侧隔室中的切割，并且异位Serrate仅诱导腹侧隔室中的切割。这些观察结果表明，给定隔室中的Notch表达细胞对Delta和Serrate具有不同的应答。我们认为Delta和Serrate在翅盘中作为隔室特异性信号发挥作用，以激活Notch并诱导翅形成所需的下游基因。
Wing margin formation in Drosophila requires the Notch receptor and, in the dorsal compartment, one of its ligands, Serrate. We provide evidence that Delta, the other known ligand for Notch, is also essential for this process. Delta is required in ventral cells at the dorsal/ventral compartment boundary, where its expression is specifically elevated in second-instar wing discs during wing margin formation. Moreover, ectopic Delta expression induces wingless, vestigial, and cut and causes adult wing tissue outgrowth in the dorsal compartment. This effect is mediated by Notch, because loss of Notch activity suppresses Delta-induced ectopic wing outgrowth. Whereas ectopic expression of Notch or the truncated activated Notch induces cut in both dorsal and ventral compartments, ectopic Delta expression induces cut only in the dorsal compartment and ectopic Serrate induces cut only in the ventral compartment. These observations indicate that Notch-expressing cells in a given compartment have different responses to Delta and Serrate. We propose that Delta and Serrate function as compartment-specific signals in the wing disc, to activate Notch and induce downstream genes required for wing formation.