Insights into the ecological roles and evolution of methyl-coenzyme M reductase-containing hot spring Archaea
Insights into the ecological roles and evolution of methyl-coenzyme M reductase-containing hot spring Archaea
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含甲基辅酶M还原酶的温泉古菌的生态作用和进化见解
DOI：
10.1038/s41467-019-12574-y
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发表时间：
2019-10-08
影响因子：
16.6
通讯作者：
Li, Wen-Jun
中科院分区：
文献类型：
--
作者：
Hua, Zheng-Shuang;Wang, Yu-Lin;Li, Wen-Jun
最近的一些研究表明，与古生物甲烷/烷烃代谢相关的关键酶--甲基辅酶M还原酶(MCR)的基因存在于与现有古菌谱系不同的元基因组组装基因组(MAG)中。在这里，我们研究了几个温泉中含有铬的古生岩浆，这揭示了执行甲烷/烷烃代谢的古生生物的多样性进一步扩大。值得注意的是，发现了一种含有微基因的MAG，该基因含有微基因，但不包括氨氧化或有氧代谢基因。综上所述，我们的系统发育分析和祖先状态重建表明，mcrABG基因在产甲烷菌和甲烷营养菌之间主要是垂直进化的，同时mcrABG基因在烷基营养菌之间的水平转移也很频繁。对含有allmcr的古菌岩浆/基因组的分析表明，根据最佳生长温度预测，这些微生物属于热液来源。这些结果还表明，甲烷/烷烃氧化或高温下的甲烷生成可能存在于共同的古生物祖先中。
Several recent studies have shown the presence of genes for the key enzyme associated with archaeal methane/alkane metabolism, methyl-coenzyme M reductase (Mcr), in metagenome-assembled genomes (MAGs) divergent to existing archaeal lineages. Here, we study themcr-containing archaeal MAGs from several hot springs, which reveal further expansion in the diversity of archaeal organisms performing methane/alkane metabolism. Significantly, an MAG basal to organisms from the phylumThaumarchaeotathat containsmcrgenes, but not those for ammonia oxidation or aerobic metabolism, is identified. Together, our phylogenetic analyses and ancestral state reconstructions suggest a mostly vertical evolution ofmcrABGgenes among methanogens and methanotrophs, along with frequent horizontal gene transfer ofmcrgenes between alkanotrophs. Analysis of allmcr-containing archaeal MAGs/genomes suggests a hydrothermal origin for these microorganisms based on optimal growth temperature predictions. These results also suggest methane/alkane oxidation or methanogenesis at high temperature likely existed in a common archaeal ancestor.