Development of a duplex lateral flow assay for simultaneous detection of antibodies against African and Classical swine fever viruses
Development of a duplex lateral flow assay for simultaneous detection of antibodies against African and Classical swine fever viruses
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DOI：
10.1177/1040638716654942
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发表时间：
2016-09-01
影响因子：
1.5
通讯作者：
Rueda, Paloma
中科院分区：
文献类型：
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作者：
Sastre, Patricia;Perez, Teresa;Rueda, Paloma
猪瘟（CSF）和非洲猪瘟（ASF）都是家猪和野猪的高度接触性疾病，临床上难以区分。对于这两种疾病，抗体检测是预防和控制措施的一个不可或缺的关键部分。本研究的目的是开发并初步验证一种用于同时检测和区分CSF病毒（CSFV）和ASF病毒（ASFV）特异性抗体的双重笔侧试验。该试验基于ASFV的主要衣壳蛋白VP72和CSFV的结构蛋白E2，两者都被认为是这些病毒的最具免疫原性的蛋白。使用一组猪样本（包括实验血清、参考血清和田间血清，后者采集自无两种疾病的欧洲农场）评价了笔旁试验的性能。新的侧流试验能够检测ASFV或CSFV的特异性抗体，显示出良好的敏感性和特异性水平。这些初步数据表明，新开发的笔旁试验用于快速鉴别检测2种疾病中发现的抗体的潜力，这在现场和一线实验室中特别重要，因为设备和熟练人员有限，ASF和CSF的控制至关重要。
Classical swine fever (CSF) and African swine fever (ASF) are both highly contagious diseases of domestic pigs and wild boar and are clinically indistinguishable. For both diseases, antibody detection is an integral and crucial part of prevention and control measures. The purpose of our study was to develop and initially validate a duplex pen-side test for simultaneous detection and differentiation of specific antibodies against CSF virus (CSFV) and ASF virus (ASFV). The test was based on the major capsid protein VP72 of ASFV and the structural protein E2 of CSFV, both considered the most immunogenic proteins of these viruses. The performance of the pen-side test was evaluated using a panel of porcine samples consisting of experimental, reference, and field sera, with the latter collected from European farms free of both diseases. The new lateral flow assay was able to detect specific antibodies to ASFV or CSFV, showing good levels of sensitivity and specificity. These preliminary data indicate the potential of the newly developed pen-side test for rapid differential detection of antibodies found in the 2 diseases, which is of particular importance in the field and in front-line laboratories where equipment and skilled personnel are limited and control of ASF and CSF is crucial.