Scaling Up Digital Circuit Computation with DNA Strand Displacement Cascades
Scaling Up Digital Circuit Computation with DNA Strand Displacement Cascades
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DOI：
10.1126/science.1200520
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发表时间：
2011-06-03
期刊：
影响因子：
56.9
通讯作者：
Winfree, Erik
中科院分区：
文献类型：
--
作者：
Qian, Lulu;Winfree, Erik
要从头开始构建复杂的生化电路，人们需要了解构建模块有多简单，以及这种电路的规模有多强大。使用一个简单的DNA反应机制的基础上的可逆链置换过程中，我们实验证明了几个数字逻辑电路，最终在一个四位平方根电路，包括130个DNA链。这些多层电路在每个逻辑操作中包括阈值和催化以执行数字信号恢复，这使得在具有大致恒定的开关时间和线性信号传播延迟的大型电路中能够实现快速和可靠的功能。该设计自然地结合了大规模电路的其他关键元素，例如通用调试工具，并行电路准备和自动电路编译器支持的抽象层次结构。
To construct sophisticated biochemical circuits from scratch, one needs to understand how simple the building blocks can be and how robustly such circuits can scale up. Using a simple DNA reaction mechanism based on a reversible strand displacement process, we experimentally demonstrated several digital logic circuits, culminating in a four-bit square-root circuit that comprises 130 DNA strands. These multilayer circuits include thresholding and catalysis within every logical operation to perform digital signal restoration, which enables fast and reliable function in large circuits with roughly constant switching time and linear signal propagation delays. The design naturally incorporates other crucial elements for large-scale circuitry, such as general debugging tools, parallel circuit preparation, and an abstraction hierarchy supported by an automated circuit compiler.