Infrared microscopic imaging of bone:: Spatial distribution of CO32-
Infrared microscopic imaging of bone:: Spatial distribution of CO32-
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DOI：
10.1359/jbmr.2001.16.5.893
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发表时间：
2001-05-01
影响因子：
6.2
通讯作者：
Mendelsohn, R
中科院分区：
文献类型：
--
作者：
Ou-Yang, H;Paschalis, EP;Mendelsohn, R
本文描述了一种利用红外 (IR) 成像以类似于 6 µm 空间分辨率定量测定骨矿物质中 CO32- 取代空间分布的新技术。这项新技术由 64 x 64 个元件组成的红外阵列探测器组成，映射到红外显微镜焦平面上的 400 m x 400 m 点。在每次扫描期间，都会从阵列中的每个元件获取完整的红外光谱。阵列中任何 IR 参数的变化都可以被映射。在目前的研究中。在一系列合成碳酸磷灰石中，在 1415 cm(-1) 处的 CO32- v, 等值线与 PO43- v(1),v(3) 等值线之间观察到的带面积或峰高比之间存在线性关系 n，光谱和分析确定的比率之间的相关系数 (R-2 = 0.989) 证明了该红外面积比在测定骨 CO32- 水平方面的实用性。这种关系构成了使用红外成像测定组织切片中 CO32- 的基础。在四幅骨小梁图像中，平均 CO32- 水平分别为 5.95 wt%（2298 个数据点）、6.67%（2040 个数据点）、6.66%（1176 个数据点）和 6.73%（2256 个数据点），总体平均值为 6.38 +/- 0.14%（7770 个数据点）。在小梁边缘和紧邻哈弗氏管处发现了最高水平的 CO32-。对合成磷灰石的磷酸盐 v(1)、v(3) 轮廓衍生的参数的检查表明，羟基磷灰石 (HA) 晶体的结晶度/完美度随着 CO32- 水平的增加而降低。所描述的方法将允许评估患病组织和正常矿化组织中CO32-水平的空间分布。
This article describes a novel technology for quantitative determination of the spatial distribution of CO32- substitution in bone mineral using infrared (IR) imaging at similar to \6 mum spatial resolution. This novel technology consists of an IR array detector of 64 x 64 elements mapped to a 400 mum x 400 mum spot at the focal plane of an IR microscope. During each scan, a complete IR spectrum is acquired from each element in the array. The variation of any IR parameter across the array may be mapped. In the current study. a linear relationship n as observed between the band area or the peak height ratio of the CO32- v, contour at 1415 cm(-1) to the PO43- v(1),v(3) contour in a series of synthetic carbonated apatites, The correlation coefficient between the spectroscopically and analytically determined ratios (R-2 = 0.989) attests to the practical utility of this IR area ratio for determination of bone CO32- levels. The relationship forms the basis for the determination of CO32- in tissue sections using IR imaging. In four images of trabecular bone the average CO32- levels were 5.95 wt% (2298 data points), 6.67% (2040 data points), 6.66% (1176 data points), and 6.73 % (2256 data points) with an overall average of 6.38 +/- 0.14% (7770 data points). The highest levels of CO32- were found at the edge of the trabeculae and immediately adjacent to the Haversian canal. Examination of parameters derived from the phosphate v(1), v(3) contour of the synthetic apatites revealed that the crystallinity/perfection of the hydroxyapatite (HA) crystals was diminished as CO32- levels increased. The methodology described will permit evaluation of the spatial distribution of CO32- levels in diseased and normal mineralized tissues.