Engineering expression of the heavy metal transporter MerC in Saccharomyces cerevisiae for increased cadmium accumulation
Engineering expression of the heavy metal transporter MerC in Saccharomyces cerevisiae for increased cadmium accumulation
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DOI：
10.1007/s00253-009-2402-0
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发表时间：
2010-03
影响因子：
5
通讯作者：
M. Kiyono;Kiyomi Miyahara;Yuka Sone;H. Pan‐Hou;Shimpei Uraguchi;Ryosuke Nakamura;K. Sakabe
中科院分区：
文献类型：
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作者：
M. Kiyono;Kiyomi Miyahara;Yuka Sone;H. Pan‐Hou;Shimpei Uraguchi;Ryosuke Nakamura;K. Sakabe
来自Tn 21编码的merC基因作为生物修复的分子工具具有潜在的用途。在酿酒酵母（Saccharomyces cerevisiae）中以融合蛋白MerC-Sso 1 p和MerC-Vam 3 p的形式表达。绿色荧光蛋白（GFP）-MerC-Sso 1 p融合蛋白主要定位于质膜，尽管在内质网中检测到一些蛋白。相反，GFP-MerC-Vam 3 p在液泡膜中表达。这些结果表明，酵母Sso 1 p和Vam 3 p是必不可少的靶向分子的质膜和液泡膜，分别。表达MerC-Sso 1 p的酵母细胞比MerC-Vam 3 p或对照细胞积累了更多的镉离子。这些结果表明，MerC在质膜上的表达可能是一个特别有前途的策略，提高镉在酵母中的积累。
ThemerCgene from the Tn21-encodedmeroperon has potential uses as a molecular tool for bioremediation. It was overexpressed as the fusion proteins MerC-Sso1p or MerC-Vam3p inSaccharomyces cerevisiae. Green fluorescent protein (GFP)-MerC-Sso1p fusion proteins located primarily in the plasma membrane, although some protein was detected in the endoplasmic reticulum. In contrast, GFP-MerC-Vam3p was expressed in the vacuolar membranes. These results suggest that yeast Sso1p and Vam3p are essential for targeting molecules to the plasma and vacuolar membranes, respectively. Significantly more cadmium ions were accumulated by yeast cells expressing MerC-Sso1p than with MerC-Vam3p or control cells. These results suggest that expression of MerC in the plasma membrane may be a particularly promising strategy for improving accumulation of cadmium in yeast.