Integrin-α5 promoted the progression of oral squamous cell carcinoma and modulated PI3K/AKT signaling pathway
Integrin-α5 promoted the progression of oral squamous cell carcinoma and modulated PI3K/AKT signaling pathway
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DOI：
10.1016/j.archoralbio.2019.03.007
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发表时间：
2019-05-01
影响因子：
3
通讯作者：
Liu, Xian-Bin
中科院分区：
文献类型：
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作者：
Fan, Qing-Chun;Tian, Hua;Liu, Xian-Bin
背景：整合素α 5（ITGA 5）基因在多种肿瘤的发生发展中起重要作用。然而，ITGA 5在口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）中的作用尚不清楚。使用siRNA构建具有沉默ITGA 5的HO 1-N-1和SCC-9细胞。采用CCK-8细胞计数法、集落形成实验、创伤愈合实验和transwell实验检测ITGA 5在口腔鳞癌细胞中的生物学功能。Western blot检测PI 3 K、p-PI 3 K、AKT、p-AKT、ERK和PERK的表达。生物学实验进一步证实ITGA 5在OSCC细胞系中表达较高。此外，我们发现ITGA 5的敲低抑制OSCC细胞的增殖、迁移和侵袭。结论：ITGA 5可通过激活PI 3 K/AKT信号通路促进OSCC的发生发展，可作为OSCC治疗的潜在生物标志物。
Background: Integrin-alpha 5 (ITGA5) gene has been reported to be critical for the progression of several cancers. However, the effects of ITGA5 in oral squamous cell carcinoma (OSCC) remain unclear.Methods: We firstly used bioinformatics methods to analyze the ITGA5 gene expression based on the public dataset. HO1-N-1 and SCC-9 cells with silenced ITGA5 were constructed using siRNA. Then, we determined the biological functions of ITGA5 in OSCC cells using cell counting kit-8 (CCK-8) assay, colony formation assay, wound healing assay and transwell assays. The expression of PI3K, p-PI3K, AKT, p-AKT, ERK and PERK were determined by western blot.Results: Our results revealed that ITGA5 expression was up-regulated in OSCC. The biological experiments further confirmed that ITGA5 expression was higher in OSCC cell lines. Moreover, we found that knockdown of ITGA5 inhibited the proliferation, migration and invasion of OSCC cells. The expression of phosphorylated-(p) PI3K, p-AKT and p-ERK obviously decreased after knockdown of ITGA5 in OSCC cells.Conclusion: In summary, ITGA5 could promote the progression of OSCC via activating the PI3K/AKT signaling pathway, and it can be regarded as a potential biomarker for OSCC treatment.