INFLUENCE OF TETRACYCLINES ON HUMAN POLYMORPHONUCLEAR LEUKOCYTE FUNCTION
INFLUENCE OF TETRACYCLINES ON HUMAN POLYMORPHONUCLEAR LEUKOCYTE FUNCTION
复制标题
DOI：
10.1128/aac.25.3.354
复制
发表时间：
1984-01-01
影响因子：
4.9
通讯作者：
SOLBERG, CO
中科院分区：
文献类型：
--
作者：
GLETTE, J;SANDBERG, S;SOLBERG, CO
低浓度土霉素、多西环素或米诺环素(10微克/毫升)不影响中性粒细胞[PMN]的随机迁移、化学发光或葡萄糖氧化。在高浓度的多西环素或米诺环素(10微克/毫升)下，细胞的化学发光和葡萄糖氧化均受到抑制。高浓度的多西环素也减少了随机迁移。土霉素在100微克/毫升时不影响这些功能，当加入4 mM的镁离子时，多西环素和米诺环素的抑制作用被消除，4 mM的钙离子部分恢复了米诺环素抑制的PMN功能。因此，四环素类药物在体外对PMN功能的主要影响是通过它们的二价阳离子螯合作用来实现的；体外实验的结果高度依赖于反应混合物中二价阳离子的浓度。四环素之间的差异可能是由于脂类溶解度的不同，其中米诺环素的溶解度最高，土霉素的溶解度最低，或者是由于二价阳离子螯合能力的不同。
Low concentrations of oxytetracycline, doxycycline or minocycline (< 10 .mu.g/ml) did not influence in vitro polymorphonuclear leukocyte [PMN] random migration, chemiluminescence or glucose oxidation. At high concentrations of doxycycline or minocycline (> 10 .mu.g/ml), chemiluminescence and glucose oxidation were impaired. High concentrations of doxycycline also reduced random migration. Oxytetracycline did not influence these functions in concentrations up to 100 .mu.g/ml. The inhibiting effect of doxycycline and minocycline was abolished when 4 mM Mg2+ was added to the reaction mixture; 4 mM Ca2+ partly restored minocycline-inhibited PMN functions. Thus, the major effect of tetracyclines on in vitro PMN functions is mediated by their divalent cation chelating effect; the results of in vitro experiments are highly dependent on the concentration of divalent cations in the reaction mixtures. The difference between the tetracyclines may be due to differences in lipid solubility, with solubility being highest for minocycline and lowest for oxytetracycline, or to different divalent cation chelating ability.