ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES.
ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES.
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DOI：
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发表时间：
1964-05
期刊：
影响因子：
3.3
通讯作者：
H. C. Douglas;D. Hawthorne
中科院分区：
文献类型：
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作者：
H. C. Douglas;D. Hawthorne
我们的酵母菌种苗中通过阻断该反应序列中的特定步骤产生半乳糖阴性表型的突变是gal，它影响半乳糖激酶的活性（ROBICHON-SZULMAJSTER 1958）和gu，它影响磷酸葡萄糖变位酶的活性（DOUGLAS 1961）。其他突变也会影响酵母的半乳糖表型。 gu突变体（DOUGLAS和CONDIE 1954，ROBICHON-SZULMAJSTER 1961）影响半乳糖向细胞内的转运，而长期适应半乳糖的表型（WINGE和ROBERTS 1948；SPIEGELMAN、SUSSMAN和PINSKA 1950）是gu突变的结果。 gu 表型的抑制是由隐性突变引起的，隐性突变允许半乳糖激酶、转移酶和差向异构酶的组成型合成（DOUGLAS 和 PELROY 1963）。目前的工作涉及突变 ga、gu 和 gal" 在半乳糖途径中的作用，以及半乳糖基因的连锁关系。将提供证据表明 gu7 和 gal" 分别阻断转移酶和差向异构酶的合成，而 gu4 则阻止所有三种半乳糖基因的合成。
Mutations in our breeding stocks of Saccharomyces that produce the galactosenegative phenotype by blocking specific steps in this reaction sequence are gal, which affects the activity of galactokinase ( ROBICHON-SZULMAJSTER 1958) and gu,, which affects the activity of phosphoglucomutase (DOUGLAS 1961). Other mutations also affect the galactose phenotype of yeast. The transport of galactose into the cell is affected in gu, mutants (DOUGLAS and CONDIE 1954, ROBICHON-SZULMAJSTER 1961 ) while the phenotype of long-term adaption to galactose (WINGE and ROBERTS 1948; SPIEGELMAN, SUSSMAN, and PINSKA 1950) is a consequence of the gu, mutation. Suppression of the gu, phenotype is brought about by the recessive mutation iwhich allows constitutive synthesis of galactokinase, transferase and epimerase (DOUGLAS and PELROY 1963). The present work concerns the roles of the mutations ga,, gu,, and gal" in the galactose pathway, and the linkage relationships of the galactose genes. Evidence will be presented to show that gu7 and gal" block the synthesis of transferase and epimerase, respectively, and that gu4 prevents the synthesis of all three of the