Characterization of the vaccinia MVA hemagglutinin gene locus and its evaluation as an insertion site for foreign genes
Characterization of the vaccinia MVA hemagglutinin gene locus and its evaluation as an insertion site for foreign genes
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DOI：
10.1016/0378-1119(96)00267-3
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发表时间：
1996-10-24
期刊：
影响因子：
3.5
通讯作者：
Dorner, F
中科院分区：
文献类型：
--
作者：
Antoine, G;Scheiflinger, F;Dorner, F
“改性安卡拉牛痘”（MVA）毒株是一种潜在的活疫苗载体。评价了MVA株血凝素（ha）基因作为外源基因插入位点的应用。为了确定MVA血凝素阴性（HA(-)）表型的分子基础，我们对HA基因及其周围区域进行了测序。氨基酸（aa）序列与其他牛痘菌株的功能性血凝素进行比较，预测其为功能性多肽。然而，ha基因的启动子区域的后期部分被删除，导致ha基因功能的明显丧失。然而，将外源DNA插入ha基因中可以产生功能性重组病毒，这表明ha基因区域是一个合适的插入位点。
The 'Modified Vaccinia Ankara' (MVA) strain is a potential live vaccine vector. The use of the hemagglutinin (ha) gene of the MVA strain as an insertion site for foreign genes was evaluated. To identify the molecular basis of the hemagglutinin-negative (HA(-)) phenotype of MVA, the ha gene and the region around this gene were sequenced. Amino acid (aa) sequence comparisons with functional hemagglutinins of other vaccinia strains predicted a functional polypeptide. The late part of the promoter region of the ha gene, however, was deleted, causing the apparent loss of the ha gene function. Nevertheless, insertion of foreign DNA into the ha gene allowed generation of functional recombinant viruses, indicating that the ha-gene region is a suitable insertion site.