Rapid and inexpensive detection of multidrug-resistant Mycobacterium tuberculosis with the nitrate reductase assay using liquid medium and direct application to sputum samples
Rapid and inexpensive detection of multidrug-resistant Mycobacterium tuberculosis with the nitrate reductase assay using liquid medium and direct application to sputum samples
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DOI：
10.1128/jcm.00083-08
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发表时间：
2008-10-01
影响因子：
9.4
通讯作者：
Portaels, Francoise
中科院分区：
文献类型：
--
作者：
Affolabi, Dissou;Odoun, Mathieu;Portaels, Francoise
为了快速、低成本地检测多药耐药结核分枝杆菌，我们采用硝酸还原酶试验（NRA），使用液体培养基直接检测痰液样本。NRA共分析了179份痰液样本，并将结果与间接比例法（IPM）作为标准参考获得的结果进行了比较。在144份具有可比结果的标本中，仅获得了一个不一致结果：NRA的MDR，但IPM的敏感性。全国步枪协会在10天内总共获得了56%的结果。在液体介质中进行的NRA是快速和廉价的，可以很容易地在低收入国家实施。
For rapid and low-cost detection of multidrug-resistant (MDR) Mycobacterium tuberculosis, we applied the nitrate reductase assay (NRA) using a liquid medium directly to sputum samples. A total of 179 sputum samples were analyzed by the NRA, and results were compared to those obtained by the indirect proportion method (IPM) as a standard reference. Out of 144 specimens for which comparable results were available, only one discrepant result was obtained: MDR by NRA but susceptible by the IPM. In total 56% of the results were obtained in 10 days by the NRA. NRA performed in liquid medium is rapid and inexpensive and can be easily implemented in low-income countries.