Influence of bone marrow-derived mesenchymal stem cells pre-implantation differentiation approach on periodontal regeneration in vivo
Influence of bone marrow-derived mesenchymal stem cells pre-implantation differentiation approach on periodontal regeneration in vivo
复制标题
骨髓间充质干细胞植入前分化方式对体内牙周再生的影响
DOI：
10.1111/jcpe.12379
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发表时间：
2015-04-01
影响因子：
6.7
通讯作者：
Walboomers, X. Frank
中科院分区：
文献类型：
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作者：
Cai, Xinjie;Yang, Fang;Walboomers, X. Frank
目的先前已证明骨髓间充质干细胞（MSC）的植入可成功实现牙周再生。然而，获得最佳牙周再生的首选植入前分化策略（例如维持干性、成骨或软骨形成诱导）仍然未知。本体内研究探讨了哪种分化方法最适合牙周再生。 材料和方法从 Fischer 大鼠中获得间充质干细胞，并将其接种到聚乳酸-乙醇酸/聚己内酯电纺支架上，然后在不同的体外条件下预培养：（i）保留多谱系分化潜力； (ii) 成骨分化方法； (iii)软骨分化方法。随后，将细胞支架结构植入 Fischer 大鼠的实验性牙周缺损中，并以空支架作为对照。种植6周后，应用组织形态计量学分析评估再生的牙周组织。结果软骨分化方法显示牙槽骨和韧带组织的再生。多系分化潜能的保留仅支持韧带再生，而成骨分化方法则促进牙槽骨再生。结论在目前使用的大鼠模型中，植入前MSCs的软骨分化是牙槽骨和牙周膜再生的有效策略。
AimThe implantation of bone marrow-derived mesenchymal stem cells (MSCs) has previously been shown successful to achieve periodontal regeneration. However, the preferred pre-implantation differentiation strategy (e.g. maintenance of stemness, osteogenic or chondrogenic induction) to obtain optimal periodontal regeneration is still unknown. This invivo study explored which differentiation approach is most suitable for periodontal regeneration.Materials and MethodsMesenchymal stem cells were obtained from Fischer rats and seeded onto poly(lactic-co-glycolic acid)/poly(-caprolactone) electrospun scaffolds, and then pre-cultured under different invitro conditions: (i) retention of multilineage differentiation potential; (ii) osteogenic differentiation approach; and (iii) chondrogenic differentiation approach. Subsequently, the cell-scaffold constructs were implanted into experimental periodontal defects of Fischer rats, with empty scaffolds as controls. After 6weeks of implantation, histomorphometrical analyses were applied to evaluate the regenerated periodontal tissues.ResultsThe chondrogenic differentiation approach showed regeneration of alveolar bone and ligament tissues. The retention of multilineage differentiation potential supported only ligament regeneration, while the osteogenic differentiation approach boosted alveolar bone regeneration.ConclusionChondrogenic differentiation of MSCs before implantation is a useful strategy for regeneration of alveolar bone and periodontal ligament, in the currently used rat model.