Modification by serum of differentiation of cultured human myeloid leukemia cells in response to 12-O-tetradecanoylphorbol-13-acetate.
Modification by serum of differentiation of cultured human myeloid leukemia cells in response to 12-O-tetradecanoylphorbol-13-acetate.
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血清对培养的人骨髓性白血病细胞响应 12-O-十四烷酰佛波醇-13-乙酸酯的分化进行修饰。
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发表时间：
1982
期刊：
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通讯作者：
M. Hozumi
中科院分区：
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作者：
Y. Honma;T. Kasukabe;M. Hozumi
在含血清的培养液中，12-O-十四酰佛波醇-13-醋酸酯(TPA)可诱导人早幼粒白血病细胞(HL-60)的α-醋酸萘酯酶活性，使其与培养皿黏附，并形成与巨噬细胞形态相似的形态。HL-60细胞在添加胰岛素、转铁蛋白和多种微量元素的无血清合成培养液中生长，并能维持6个月以上。在无血清培养条件下观察TPA对细胞的诱导分化作用。有血清培养的人髓系白血病细胞(K562-4)即使在有TPA的情况下也不能被诱导分化，但在无血清培养4个月后，在TPA、精氨酸酶或放线菌素D的作用下，观察到它们向巨噬细胞的分化。加入血清可抑制在无血清条件下培养的HL-60和K562-4细胞的分化。小牛血清对TPA诱导分化的抑制作用强于胎牛血清，但两者对放线菌素D和精氨酸酶诱导分化的抑制作用无显著差异。这些结果表明，不同血清在不同介质中的不同反应可能是针对TPA的。TPA诱导K562-4细胞黏附需要一些未知的血清因素(S)，尽管血清的加入抑制了K562-4细胞的形态和功能分化的诱导。本文讨论了K-562-4细胞诱导分化为巨噬细胞的能力与长期无血清培养的关系。
In medium with serum, 12-O-tetradecanoylphorbol-13-acetate (TPA) induced alpha-naphthyl acetate esterase activity in human promyelocytic leukemia cells (HL-60), adherence of the cells to the culture dish, and their change into forms that were morphologically similar to macrophages. HL-60 cells grew in the absence of serum in synthetic medium supplemented with insulin, transferrin, and several trace elements, and could be maintained for more than 6 months in this medium. Induction of differentiation by TPA was observed with cells grown in serum-free medium. Human myeloid leukemia cells (K562-4) cultured in medium with serum could not be induced to differentiate even in the presence of TPA, but their differentiation into macrophages in the presence of TPA, arginase or actinomycin D was observed after they had been grown in serum-free medium for 4 months. Addition of serum inhibited the induction of differentiation of HL-60 and K562-4 cells that had been grown in serum-free medium. Calf serum was more inhibitory than fetal calf serum on TPA-induced differentiation, but there was no significant difference in the effects of the two sera on induction by actinomycin D or arginase. These results suggest that the different responses in media with different sera may be specific to TPA. Induction of adhesiveness of K562-4 cells by TPA required some unknown serum factor(s), although addition of serum inhibited the inductions of morphological and functional differentiation. The relation between the ability of K-562-4 cells to be induced to differentiate into macrophages and long-term cultivation in serum-free medium is discussed.