PROTEIN-SYNTHESIS IN SALIVARY-GLANDS OF DROSOPHILA-MELANOGASTER - RELATION TO CHROMOSOME PUFFS
PROTEIN-SYNTHESIS IN SALIVARY-GLANDS OF DROSOPHILA-MELANOGASTER - RELATION TO CHROMOSOME PUFFS
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DOI：
10.1016/0022-2836(74)90447-1
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发表时间：
1974-01-01
影响因子：
5.6
通讯作者：
TRACY, UM
中科院分区：
文献类型：
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作者：
TISSIERES, A;MITCHELL, HK;TRACY, UM
在整个蛹前期，以及热休克和蜕皮甾酮处理后的不同时间解剖果蝇的唾液腺和其他组织，并用[35 S]蛋氨酸孵育分离的组织标记的蛋白质通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离。从唾液腺的标记带图案，如所看到的凝胶的放射自显影，显示出惊人的变化，在一种方式非常相似的变化，在同一prepupal期间的粉扑模式。在马氏管蛋白质中，谱带的变化范围较小，在脑中只有少数组分发生了改变，热休克引起了一些新谱带的出现，而其他谱带的强度则降低。在所有检查的组织，唾液腺，脑和马氏管，以及翅成虫盘，缺乏多线染色体的组织中观察到这种效果。由热休克诱导的六个最重标记的条带占样品中总标记的约30%，一个组分单独占15%以上，如在唾液腺、脑和马氏管中所见。用[3 H]尿苷标记法研究了热激处理后在喷烟部位的RNA合成。通过将某些喷烟中[3 H]尿苷的量与某些条带中[35 S]蛋氨酸的水平相关联，在少数情况下提出了一种初步的关系。注意到一些蛋白质条带的变化，尽管它们比热休克后的变化要小得多。
The salivary glands and other tissues fromDrosophila melanogasterwere dissected at various times throughout the prepupal period, as well as after heat shocks and ecdysterone treatments, and the proteins labelled by incubating the isolated tissues with [35S]methionine were separated by electrophoresis on sodium dodecyl sulphate-polyacrylamide gel. The labelled band patterns from salivary gland, as seen on the autoradiograph of the gel, showed striking variations, in a manner remarkably similar to variations in puff patterns during the same prepupal period. In proteins from Malpighian tubes, the pattern of bands varied to a lesser extent and in brain only a few components were modified.Heat shock brought about the appearance of a number of new bands, while others were reduced in intensity. This effect was observed with all the tissues examined, salivary glands, brain and Malpighian tubes, as well as wing imaginal discs, tissue lacking polytene chromosomes. The six most heavily labelled bands induced by heat shock represent about 30%, and one component alone represents over 15%, of the total label in the sample, as seen in salivary glands, brain and Malpighian tubes. The synthesis of RNA at puff sites was investigated after heat shock by [3H]uridine labelling. By correlating the amount of [3H]uridine in some puffs with the level of [35S]methionine in some bands a tentative relation is suggested in a few instances.The effect of ecdysterone treatment was also studied in the salivary glands. Changes in a number of protein bands were noticed, though they were much less pronounced than those following heat shock.