Microglial and astrocytic changes in the striatum of methamphetamine abusers
Microglial and astrocytic changes in the striatum of methamphetamine abusers
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DOI：
10.1016/j.legalmed.2009.11.001
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发表时间：
2010-03-01
期刊：
影响因子：
1.5
通讯作者：
Kubo, Shin-ichi
中科院分区：
文献类型：
--
作者：
Kitamura, Osamu;Takeichi, Toshiaki;Kubo, Shin-ichi
关于慢性甲基苯丙胺（METH）使用者纹状体中神经胶质细胞的作用知之甚少。在这项研究中，我们用化学方法检测了长期使用METH而死于药物中毒的METH使用者纹状体中的神经胶质反应。人葡萄糖转运蛋白5（hGLUT），小胶质细胞的有用标志物，和CR3.43，反应性小胶质细胞特异性的主要组织相容性复合物II类抗原，进行免疫染色。胶质细胞酸性蛋白（GFAP）和S100 B用于星形胶质细胞免疫组化。我们分析了12名慢性METH使用者和13名对照受试者，并检测到慢性METH使用者中hGLUT 5阳性细胞数量增加了200-240%（p < 0.01）。然而，我们没有检测到CR3.43阳性细胞的任何增殖。GFAP阳性星形胶质细胞数量增加，但增加不显著（p > 0.05）。两组间S100 B阳性细胞密度差异无统计学意义（p > 0.05）。这项研究表明，长期使用METH的人纹状体中没有反应性胶质增生，他们没有长时间使用METH。结果表明，长期使用METH本身不会激活人类的神经胶质细胞，反应性神经胶质增生可能不参与药物摄入失控的机制，这是药物成瘾的一个特征。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Little is known about the role of glial cells in the striatum of chronic methamphetamine (METH) users. In this study, we immunohistochemically examined glial reactions in the striatum of chronic METH users who did not abstain from METH use and died of drug intoxication. Human glucose transporter 5 (hGLUT), a useful marker of microglia, and CR3.43, a major histocompatibility complex class II antigen specific for reactive microglia, were immunostained. Glial fibrillary acidic protein (GFAP) and S100B were used for astrocyte immunohistochemistry. We analyzed 12 chronic METH users and 13 control subjects, and detected a 200-240% increase in the number of hGLUT5-positive cells in chronic METH users (p < 0.01). However, we did not detect any proliferation of CR3.43-positive cells. The number of GFAP-positive astrocytes increased, but this increase was not significant (p > 0.05). Moreover, S100B-positive cell density between the two groups was not significant (p > 0.05). This study demonstrates the absence of reactive gliosis in the striatum of chronic METH users who did not abstain for prolonged periods from METH use. The results suggest that chronic METH use by itself did not activate glial cells in humans and reactive gliosis may not be involved in the mechanism underlying the loss of control in drug intake, which is a characteristic feature of drug addiction. (C) 2009 Elsevier Ireland Ltd. All rights reserved.