REGULATION OF RESERVE PROTEIN-METABOLISM IN COTYLEDONS OF MUNG BEAN SEEDLINGS
REGULATION OF RESERVE PROTEIN-METABOLISM IN COTYLEDONS OF MUNG BEAN SEEDLINGS
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DOI：
10.1073/pnas.73.9.3168
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
HARRIS, N
中科院分区：
文献类型：
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作者：
CHRISPEELS, MJ;BAUMGARTNER, B;HARRIS, N
绿豆（Phaseolus aureus，Roxb.）伴随着储备蛋白的代谢和子叶中具有内肽酶活性的蛋白水解酶的出现。在生长的前5天，酶活性增加25倍。子叶提取物制备的种子吸水24小时不与兔脱肽酶抗血清反应，这表明该酶是不存在于种子中作为酶原。标记实验表明，该酶在幼苗生长过程中合成。内肽酶位于蛋白体中，并且在这些细胞器中酶的比活性增加30倍。超微结构研究表明，粗面内质网增生，并可能产生小泡，小泡在储备蛋白消化前与蛋白体融合。这些囊泡可能是将酶从合成位点转运到作用位点的主要溶酶体。
Seedling growth in mung beans (Phaseolus aureus, Roxb.) is accompanied by the metabolism of the reserve proteins and the appearance in the cotyledons of a proteolytic enzyme with endopeptidase activity. Enzyme activity increases 25-fold during the 1st 5 days of growth. Cotyledon extracts prepared from seeds imbibed for 24 h with water do not react with rabbit dedopeptidase antiserum, which suggests that the enzyme is not present in the seeds as a zymogen. Labeling experiments show that the enzyme is synthesized in the course of seedling growth. The endopeptidase is localized in the protein bodies, and the specific activity of the enzyme in these organellex increases 30 fold. Ultrastructural studies show that the rough endoplasmic reticulum proliferates and may give ris to vesicles which fuse with the protein bodies prior to reserve protein digestion. These vesicles could be the primry lysosomes which transport the enzyme from its site of synthesis to its site of action .