Utility of the polymerase chain reaction in detection of Trypanosoma cruzi in Guatemalan Chagas' disease vectors
Utility of the polymerase chain reaction in detection of Trypanosoma cruzi in Guatemalan Chagas' disease vectors
复制标题
DOI：
10.4269/ajtmh.1999.60.740
复制
发表时间：
1999-05-01
影响因子：
3.3
通讯作者：
Monroy, C
中科院分区：
文献类型：
--
作者：
Dorn, PL;Engelke, D;Monroy, C
对于有效的控制计划，准确评估媒介中的克氏锥虫感染至关重要，传统上是通过显微镜检查进行的。对于特定载体而非其他载体，粪便样本的聚合酶链反应 (PCR) 分析最近已被证明是一种有效的检测手段。比较了危地马拉两种主要媒介锥蝽和长红锥蝽中不同解剖部位克氏锥虫的 PCR 和显微镜检测的灵敏度。初步研究表明，在 90% T. rangeli 存在的情况下，可以通过 PCR 检测到 T. cruzi。收集了一百三十五个载体，并从直肠、肠和胃中获取样本，并通过显微镜和 PCR 进行分析。对于锥蝽直肠样本，PCR 灵敏度（39.1% 克氏锥蝽阳性）和显微镜灵敏度（24.6% 阳性）没有显着差异。然而，在 R. prolixus 中，PCR 被证明比显微镜检查更敏感：PCR 的阳性率为 57.6%，而显微镜检查的阳性率为 22.7%。直肠样本的感染率最高，其次是肠道和胃样本。然而，如果仅分析直肠样本，就会漏掉 10.5% 的罗德尼乌斯感染。因此，对于检测 R. prolixus 中的 T. cruzi，PCR 明显比显微镜更灵敏。为了准确评估特定媒介的感染，除了直肠样本外，还可能需要对解剖部位进行分析。
For effective control programs, accurate assessment of Trypanosoma cruzi infection in vectors is essential and has traditionally been performed by microscopic examination. For particular vectors and not others, polymerase chain reaction (PCR) analysis of fecal samples recently has been shown to be an effective means of detection. The sensitivities of the PCR and microscopy for detection of T. cruzi in different anatomic sites were compared in the two major vectors of Guatemala, Triatoma dimidiata and Rhodnius prolixus. Preliminary studies established that T. cruzi can be detected by the PCR in the presence of 90% T. rangeli. One hundred thirty-five vectors were collected, and samples were obtained from the rectum, intestines, and stomach and analyzed by microscopy and the PCR. For Triatoma dimidiata rectal samples, the PCR sensitivity (39.1% T. cruzi positive) and the microscopic sensitivity (24.6% positive) was not significantly different. However, in R. prolixus, the PCR proved significantly more sensitive than microscopy: 57.6% positive by PCR compared with 22.7% by microscopy. Rectal samples showed the highest rates of infection followed by intestine and stomach samples. However, 10.5% of the Rhodnius infections would have been missed if only the rectal sample had been analyzed. Thus, the PCR is significantly more sensitive than microscopy for detection of T. cruzi in R. prolixus. Analysis of anatomic sites in addition to the rectal sample may be necessary for accurate assessment of infection in particular vectors.