High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand
High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand
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DOI：
10.1002/jmv.25591
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发表时间：
2020-02
影响因子：
12.7
通讯作者：
R. Tacharoenmuang;S. Komoto;R. Guntapong;Sompong Upachai;Phakapun Singchai;Tomihiko Ide;Saori Fukuda;Kriangsak Ruchusatsawast;Busarawan Sriwantana;M. Tatsumi;K. Motomura;N. Takeda;T. Murata;S. Sangkitporn;K. Taniguchi;T. Yoshikawa
中科院分区：
文献类型：
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作者：
R. Tacharoenmuang;S. Komoto;R. Guntapong;Sompong Upachai;Phakapun Singchai;Tomihiko Ide;Saori Fukuda;Kriangsak Ruchusatsawast;Busarawan Sriwantana;M. Tatsumi;K. Motomura;N. Takeda;T. Murata;S. Sangkitporn;K. Taniguchi;T. Yoshikawa
A组轮状病毒(RVA)是全球婴幼儿急性胃肠炎的主要原因。本研究旨在阐明泰国RVA流行的G/P型分布和遗传特征。2014年1月至2016年9月，从泰国6个省的儿童和成人急性胃肠炎患者身上采集了1867份粪便标本。514/1867例(27.5%)粪便标本中检出RVA。G1P[8](44.7%)是最主要的基因型，其次是G3P[8](33.7%)、G2P[4](11.5%)、G8P[8](7.0%)和G9P[8](1.3%)。G3P[9](0.8%)、G3P[10](0.4%)、G4P[6](0.4%)和G10P[14](0.2%)也在低频段出现异常。2014年的优势基因G1P[8](64.4%)下降到2016年的6.1%。相比之下，G3P[8]的频率从2014年的5.5%明显增加到2015年的65.3%和2016年的89.8%。在聚丙烯酰胺凝胶电泳法上，大多数(135/140；96.4%)G3P[8]菌株显示出较短的RNA图谱。对98株G3P[8]毒株的VP7基因核苷酸序列的测定结果表明，它们都是类马的G3P[8]毒株。在对两个具有代表性的泰国类马G3P[8]毒株基因组片段的系统发育分析中，值得注意的是，它们具有不同的nsp4基因，一个类似牛，另一个类似人。因此，我们发现具有短RNA电泳型的特征类似马的G3P[8]菌株正在泰国的儿童和成人中高度流行。
Group A rotavirus (RVA) is a major cause of acute gastroenteritis in infants and young children worldwide. This study aims to clarify the distribution of G/P types and genetic characteristics of RVAs circulating in Thailand. Between January 2014 and September 2016, 1867 stool specimens were collected from children and adults with acute gastroenteritis in six provinces in Thailand. RVAs were detected in 514/1867 (27.5%) stool specimens. G1P[8] (44.7%) was the most predominant genotype, followed by G3P[8] (33.7%), G2P[4] (11.5%), G8P[8] (7.0%), and G9P[8] (1.3%). Unusual G3P[9] (0.8%), G3P[10] (0.4%), G4P[6] (0.4%), and G10P[14] (0.2%) were also detected at low frequencies. The predominant genotype, G1P[8] (64.4%), in 2014 decreased to 6.1% in 2016. In contrast, the frequency of G3P[8] markedly increased from 5.5% in 2014 to 65.3% in 2015 and 89.8% in 2016. On polyacrylamide gel electrophoresis, most (135/140; 96.4%) of the G3P[8] strains exhibited a short RNA profile. Successful determination of the nucleotide sequences of the VP7 genes of 98 G3P[8] strains with a short RNA profile showed that they are all equine‐like G3P[8] strains. On phylogenetic analysis of genome segments of two representative Thai equine‐like G3P[8] strains, it was noteworthy that they possessed distinct NSP4 genes, one bovine‐like and the other human‐like. Thus, we found that characteristic equine‐like G3P[8] strains with a short RNA electropherotype are becoming highly prevalent in children and adults in Thailand.