Pedunculopontine glutamatergic neurons control spike patterning in substantia nigra dopaminergic neurons.
Pedunculopontine glutamatergic neurons control spike patterning in substantia nigra dopaminergic neurons.
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DOI：
10.7554/elife.30352
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发表时间：
2017-10-05
期刊：
影响因子：
7.7
通讯作者：
Surmeier DJ
中科院分区：
文献类型：
--
作者：
Galtieri DJ;Estep CM;Wokosin DL;Traynelis S;Surmeier DJ
黑质致密部(SNC)多巴胺能神经元的爆发峰是控制目标定向行为的回路中的关键信号事件。人们普遍认为，这种放电模式依赖于N-甲基-D-天冬氨酸受体(NMDAR)的突触激活和内在振荡机制之间的相互作用。然而，特定的神经网络在突发产生中的作用还没有确定。为了填补这一空白，我们用光学和电生理学方法对来自脚丘脑核(PPN)神经元的SNC谷氨酸能突触进行了表征。这些突触仅定位在胞体和近端的树突上，将它们放置在一个良好的位置，以影响棘波的产生。事实上，光遗传刺激PPN轴突可以可靠地诱发黑质多巴胺能神经元的放电。此外，即使在NMDAR拮抗剂存在的情况下，对PPN轴突的爆发性刺激也得到了忠实的遵循。因此，PPN诱发的体内黑质多巴胺能神经元的爆发峰电位不仅可能是外源性触发的，而且也可能是外源性模式的。
Burst spiking in substantia nigra pars compacta (SNc) dopaminergic neurons is a key signaling event in the circuitry controlling goal-directed behavior. It is widely believed that this spiking mode depends upon an interaction between synaptic activation of N-methyl-D-aspartate receptors (NMDARs) and intrinsic oscillatory mechanisms. However, the role of specific neural networks in burst generation has not been defined. To begin filling this gap, SNc glutamatergic synapses arising from pedunculopotine nucleus (PPN) neurons were characterized using optical and electrophysiological approaches. These synapses were localized exclusively on the soma and proximal dendrites, placing them in a good location to influence spike generation. Indeed, optogenetic stimulation of PPN axons reliably evoked spiking in SNc dopaminergic neurons. Moreover, burst stimulation of PPN axons was faithfully followed, even in the presence of NMDAR antagonists. Thus, PPN-evoked burst spiking of SNc dopaminergic neurons in vivo may not only be extrinsically triggered, but extrinsically patterned as well.
影响因子：
3.7
作者：
Ha J;Kuznetsov A
通讯作者：
Kuznetsov A