Coaggregation of Candida albicans with oral Fusobacterium species
Coaggregation of Candida albicans with oral Fusobacterium species
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DOI：
10.1111/j.1399-302x.1997.tb00374.x
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发表时间：
1997-06-01
影响因子：
--
通讯作者：
Nesbitt, WE
中科院分区：
文献类型：
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作者：
Grimaudo, NJ;Nesbitt, WE
研究了9株口腔梭杆菌与4株口腔酵母菌白色念珠菌体外共聚集的能力。所有的核梭杆菌菌株、牙周梭杆菌和沟梭杆菌都与所有的念珠菌菌株有不同程度的聚集。假丝酵母菌、紫梭杆菌和相似梭杆菌均未与假丝酵母菌共聚集。将聚集的梭杆菌菌株而非念珠菌菌株暴露于高温、胰蛋白酶和蛋白酶K中可消除共聚集。两性霉素B或木霉素对酵母菌没有影响。添加0.1 M甘露糖、氨基葡萄糖和α -甲基甘露糖甙仅能抑制反应。十二烷基硫酸钠对聚结对均有分解作用，而非离子型洗涤剂对聚结对均无影响。2.0 M尿素的加入完全逆转了共聚集。念珠菌菌株对高碘酸盐氧化敏感，而梭杆菌菌株对这种处理稳定。所有聚集都发生在有唾液存在的情况下，并且比在缓冲液中更强。这些数据表明，梭杆菌表面的蛋白或糖蛋白可能与念珠菌表面的碳水化合物或含碳水化合物的分子相互作用。这些观察结果扩大了已知的人类口腔微生物属间共聚集的范围，并表明白色梭杆菌种的共聚集可能是该酵母口腔定植的重要因素。作者认为这是第一次描述酵母细胞上的碳水化合物成分和口腔细菌细胞上的蛋白质成分的共聚集。
Nine strains of oral Fusobacterium were examined for their ability to coaggregate in vitro with four strains of the oral yeast, Candida albicans. All of the Fusobacterium nucleatum strains and Fusobacterium periodontium and Fusobacterium sulci coaggregated to various degrees with all of the Candida strains. Fusobacterium alocis, Fusobacterium mortiferum and Fusobactrium simiae strains did not coaggregate with any of the Candida strains. Exposure of the coaggregating Fusobacterium strains but not the Candida strains to heat, trypsin, and proteinase K eliminated coaggregation. Amphotericin B or trichodermin treatment of the yeast species had no effect. The reactions mere inhibited by addition of 0.1 M mannose, glucosamine and alpha-methyl mannoside. All coaggregating pairs were disaggregated by the addition of sodium dodecyl sulfate, but nonionic detergents had no effect. The addition of 2.0 M urea completely reversed coaggregation. Candida strains were sensitive to periodate oxidation, whereas the Fusobacterium strains were stable to this treatment. All coaggregations occurred in the presence of saliva and appeared stronger than in buffer. These data suggest that the coaggregations involve either a protein or glycoprotein on the Fusobacterium surface, which may interact with carbohydrates or carbohydrate-containing molecules on the surface of the Candida. These observations expand the known range of intergeneric coaggregations occurring between human oral microbes and indicate that coaggregation of C. albicans Fusobacterium species may be an important Factor in oral colonization by this yeast. The authors believe this to be the first description of coaggregation concerning a carbohydrate component on the yeast cell and a protein component on the oral bacterial cell.