MicroRNA-155-5p is associated with oral squamous cell carcinoma metastasis and poor prognosis
MicroRNA-155-5p is associated with oral squamous cell carcinoma metastasis and poor prognosis
复制标题
DOI：
10.1111/jop.12351
复制
发表时间：
2016-04-01
影响因子：
3.3
通讯作者：
Bukawa, Hiroki
中科院分区：
文献类型：
--
作者：
Baba, Osamu;Hasegawa, Shogo;Bukawa, Hiroki
研究背景近年来发现口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）中miRNA的异常表达与肿瘤的转移和预后有关。侵袭-转移级联反应的启动涉及上皮-间质转化（EMT）。我们的目的是澄清如何miRNA，特别是miR-155- 5 p的错误表达有助于OSCC转移通过EMT.MethodsWe收集肿瘤样本从73例OSCC。通过定量逆转录聚合酶链反应（qRT-PCR）分析样本，并研究miR-155- 5 p水平与临床特征之间的相关性。通过miRNA微阵列和通过用miR-155- 5 p模拟物或抑制剂转染，随后通过增殖和伤口愈合迁移测定来分析OSCC细胞系。qRT-PCR分析的EMT标志物在细胞转染miR-155- 5 p inhibitor performed.ResultsWe发现高miR-155 - 5 p表达的组织样本中的受试者与口腔鳞癌已转移到颈部淋巴结。HSC-3细胞也强烈表达miR-155- 5 p。上皮标志物E-cadherin在转染miR-155- 5 p抑制剂的HSC-3细胞中强烈表达，我们观察到这些细胞中SOCS 1的表达升高和STAT 3的表达降低。
BackgroundAbnormal miRNA expression was recently implicated in the metastasis of oral squamous cell carcinoma (OSCC) and with a poor prognosis. The initiation of the invasion-metastasis cascade involves epithelial-mesenchymal transition (EMT). Our aim was to clarify how miRNA, especially miR-155-5p misexpression contributes to OSCC metastasis through EMT.MethodsWe collected tumor samples from 73 subjects with OSCC. The samples were analyzed by quantitative reverse-transcription polymerase chain reaction (qRT-PCR), and correlations between miR-155-5p levels and clinical characteristics were investigated. OSCC cell lines were analyzed by miRNA microarray and by transfection with a miR-155-5p mimic or inhibitor, followed by proliferation and wound-healing migration assays. qRT-PCR analyses of EMT makers in cells transfected with miR-155-5p inhibitor were performed.ResultsWe found high miR-155-5p expression in tissue samples from subjects with OSCC that had metastasized to cervical lymph nodes. HSC-3 cells also strongly expressed miR-155-5p. The epithelial marker E-cadherin was strongly expressed in HSC-3 cells transfected with miR-155-5p inhibitor, and we observed elevated SOCS1 and decreased STAT3 expression in these cells.ConclusionsOur results suggest that miR-155-5p causes OSCC to metastasize, and could serve as a novel therapeutic target for OSCC.