Evaluation of the dual-precipitation method for determination of cholesterol in high-density lipoprotein subfractions HDL2 and HDL3 in serum.
Evaluation of the dual-precipitation method for determination of cholesterol in high-density lipoprotein subfractions HDL2 and HDL3 in serum.
复制标题
双沉淀法测定血清中高密度脂蛋白亚组分 HDL2 和 HDL3 胆固醇的评价。
DOI：
10.1093/clinchem/32.5.819
复制
发表时间：
1986
影响因子：
9.3
通讯作者：
H. Baadenhuysen
中科院分区：
文献类型：
--
作者：
P. Demacker;H. Hak;A. Hijmans;H. Baadenhuysen
我们比较了用于测量高密度脂蛋白亚组分HDL 2和HDL 3中胆固醇的双沉淀法（Gidez等人，J Lipid Res 1982;23：1206-33），在摆桶转子中进行密度梯度超离心（Demacker等人，Clin Chem 1983;29：656-63）。根据经验确定了HDL 2-chol和HDL 3-chol值最佳准确度所需的硫酸葡聚糖浓度15，000（DS）。在DS浓度为0.87 g/L时，88份血清中HDL 2-chol和HDL 3-chol的值与超离心法所得值无显著差异。该方法的精密度令人满意，并与浓度有关。然而，双重沉淀法缺乏特异性，因为它不产生仅含有一种HDL亚组分的组分。HDL 2和HDL 3各自含有彼此等量的胆固醇。在DS浓度增加时，一些放射性标记的HDL 3似乎在HDL 2完全沉淀之前沉淀。这种缺乏特异性的情况在大规模流行病学研究中可以接受，但在小规模干预研究或临床样本分析中则不能接受，因为这些研究需要更好的准确性，并且优选超离心。
We compared the dual-precipitation method for measurement of cholesterol in high-density lipoprotein subfractions HDL2 and HDL3 (Gidez et al., J Lipid Res 1982;23:1206-33) with density-gradient ultracentrifugation in a swinging-bucket rotor (Demacker et al., Clin Chem 1983;29:656-63). The concentration of dextran sulfate 15,000 (DS) needed for optimal accuracy of the HDL2-chol and HDL3-chol values was established empirically. At a DS concentration of 0.87 g/L, the values for HDL2-chol as well as for HDL3-chol in 88 sera did not differ significantly from those obtained by ultracentrifugation. The precision of the method was satisfactory and was related to the concentration. Nevertheless, the dual-precipitation method lacks specificity inasmuch as it produces no fractions that contain only one HDL subfraction. HDL2 and HDL3 each contained an equivalent amount of cholesterol from the other. At increasing DS concentrations, some radiolabeled HDL3 appeared to have precipitated prior to complete precipitation of HDL2. This lack of specificity can be tolerated in large-scale epidemiological studies for screening, but not in small-scale intervention studies or in assay of clinical samples, where better accuracy is needed and ultracentrifugation is preferred.