Release of outer membrane vesicles from Bordetella pertussis
Release of outer membrane vesicles from Bordetella pertussis
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DOI：
10.1007/pl00006801
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发表时间：
1999-05-01
影响因子：
2.6
通讯作者：
Yantorno, O
中科院分区：
文献类型：
--
作者：
Hozbor, D;Rodriguez, ME;Yantorno, O
本研究的目的是研究百日咳杆菌外膜囊泡（OMV）的产生。观察到许多从生长在Scholter-Scholte液体培养基中的细胞释放的囊泡。类似囊泡样结构的形成也可以通过超声处理浓缩的细菌悬浮液来人工诱导。免疫印迹分析表明，OMV含有腺苷酸环化酶-溶血素（AC-Hly），以及其他多肽，以及脂多糖（LPS）。使用纯化的AC-Hly和从B分离的OMV进行实验。百日咳AC-Hly（-）表明AC-Hly是囊泡的组成部分。本文报道的OMV含有多种保护性免疫原，可能被认为是开发无细胞百日咳疫苗的可能基础材料。
The aim of the study reported here was to investigate the production of Bordetella pertussis outer membrane vesicles (OMVs). Numerous vesicles released from cells grown in Stainer-Scholte liquid medium were observed. The formation of similar vesicle-like structures could also be artificially induced by sonication of concentrated bacterial suspensions. Immunoblot analysis showed that OMVs contain adenylate cyclase-hemolysin (AC-Hly), among other polypeptides, as well as the lipopolysaccharide (LPS). Experiments carried out employing purified AC-Hly and OMVs isolated from B. pertussis AC-Hly(-) showed that AC-Hly is an integral component of the vesicles. OMVs reported here contain several protective immunogens and might be considered a possible basic material for the development of acellular pertussis vaccines.