Comparative characterization of stem cells from human exfoliated deciduous teeth, dental pulp, and bone marrow-derived mesenchymal stem cells
Comparative characterization of stem cells from human exfoliated deciduous teeth, dental pulp, and bone marrow-derived mesenchymal stem cells
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DOI：
10.1016/j.bbrc.2018.04.213
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发表时间：
2018-06-18
影响因子：
3.1
通讯作者：
Tanimoto, Kotaro
中科院分区：
文献类型：
--
作者：
Kunimatsu, Ryo;Nakajima, Kengo;Tanimoto, Kotaro
目的：间充质干细胞（Mesenchymal stem cells，MSCs）在组织工程和再生医学领域有着广泛的应用前景. MSC的增殖和成骨分化潜能根据诸如组织来源和细胞群体异质性的因素而变化。牙齿组织作为一种容易获得的高质量干细胞来源而受到关注。本研究比较了乳牙牙髓干细胞（SHED）、人牙髓干细胞（hDPSC 5）和人骨髓间充质干细胞（hBMSCs）的体外特性。使用溴脱氧尿苷免疫测定和细胞计数分析培养细胞的增殖能力。监测碱性磷酸酶（ALP）水平以评估成骨分化。茜素红染色评价矿化。结果：SHED细胞增殖能力明显高于hDPSCs和hBMSC 5。诱导成骨分化后第21天，SHED、hDPSC 5和hBMSCs均呈现深茜素红染色，分光光度法检测hBMSCs的染色明显高于SHED和hDPSC。与SHED和hDPSC相比，hBMSC 5中的ALP染色更强，并且与SHED或hDPSC 5相比，hBMSC 5中的ALP活性显著更高。实时荧光定量PCR分析显示，与hBMSCs相比，SHED显示Runx 2和ALP基因的表达显著更高。结论：SHED细胞具有较强的增殖活性，bFGF和BMP-2基因表达水平高于BMMSC 5和DPSCs。易于收获细胞和避免侵入性外科手术的能力表明，SHED可能是用于骨再生治疗的有用细胞来源。(C)2018爱思唯尔公司All rights reserved.
Objectives: Mesenchymal stem cells (MSCs) are used clinically in tissue engineering and regenerative medicine. The proliferation and osteogenic differentiation potential of MSCs vary according to factors such as tissue source and cell population heterogeneity. Dental tissue has received attention as an easily accessible source of high-quality stem cells. In this study, we compared the in vitro characteristics of dental pulp stem cells from deciduous teeth (SHED), human dental pulp stem cells (hDPSC5), and human bone marrow mesenchymal stem cells (hBMSCs).Materials and methods: SEHD and hDPSCs were isolated from dental pulp and analyzed in comparison with human bone marrow (hBM)MSC5. Proliferative capacity of cultured cells was analyzed using a bromodeoxyuridine immunoassay and cell counting. Alkaline phosphatase (ALP) levels were monitored to assess osteogenic differentiation. Mineralization was evaluated by alizarin red staining. Levels of bone marker mRNA were examined by real-time PCR analysis.Results: SHED were highly proliferative compared with hDPSCs and hBMSC5. SHED, hDPSC5, and hBMSCs exhibited dark alizarin red staining on day 21 after induction of osteogenic differentiation, and staining of hBMSCs was significantly higher than that of SHED and hDPSCs by spectrophotometry. ALP staining was stronger in hBMSC5 compared with SHED and hDPSCs, and ALP activity was significantly higher in hBMSC5 compared with SHED or hDPSC5. SHED showed significantly higher expression of the Runx2 and ALP genes compared with hBMSCs, based on real-time PCR analysis. In bFGF, SHED showed significantly higher expression of the basic fibroblast growth factor (bFGF) gene compared with hDPSC5 and hBMSCs.Conclusion: SHED exhibited higher proliferative activity and levels of bFGF and BMP-2 gene expression compared with BMMSC5 and DPSCs. The ease of harvesting cells and ability to avoid invasive surgical procedures suggest that SHED may be a useful cell source for application in bone regeneration treatments. (C) 2018 Elsevier Inc. All rights reserved.