Navigation to the graveyard-induction of various pathways of necrosis and their classification by flow cytometry.
Navigation to the graveyard-induction of various pathways of necrosis and their classification by flow cytometry.
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DOI：
10.1007/978-1-62703-383-1_1
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发表时间：
2013-01-01
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影响因子：
--
通讯作者：
Herrmann, Martin
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文献类型：
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作者：
Janko, Christina;Munoz, Luis;Herrmann, Martin
凋亡和坏死分别反映了垂死细胞所采用的细胞死亡程序和死亡的最后阶段。细胞凋亡被定义为一种生理性的、高度组织化的细胞死亡过程，而坏死通常被认为是偶然的和不受控制的。生理性和弱的病理性死亡刺激优先诱导细胞凋亡，而苛刻的非生理性损伤往往立即煽动（原发性）坏死。如果一个凋亡细胞过渡到一个阶段的质膜解体，这一阶段的死亡被称为继发性或凋亡后necrosis.在这里，我们提出了几个条件，刺激原发性和/或继发性坏死，并显示坏死显示相当不同的时间进程。对于坏死表型的亚分类，我们采用了流式细胞术单管四色染色技术，包括膜联蛋白A5-FITC、碘化丙啶、DiIC 1（5）和Hoechst 33342。
Apoptosis and necrosis reflect the program of cell death employed by a dying cell and the final stage of death, respectively. Whereas apoptosis is defined as a physiological, highly organized cell death process, necrosis is commonly considered to be accidental and uncontrolled. Physiological and weak pathological death stimuli preferentially induce apoptosis, while harsh non-physiological insults often immediately instigate (primary) necrosis. If an apoptosing cell transits into a phase of plasma membrane disintegration, this stage of death is referred to as secondary or post-apoptotic necrosis.Here, we present several conditions that stimulate primary and/or secondary necrosis and show that necrosis displays considerably different time courses. For subclassification of necrotic phenotypes we employed a flow cytometric single-tube 4-color staining technique including annexin A5-FITC, propidium iodide, DiIC1(5), and Hoechst 33342.