CELL-CYCLE REGULATION IN MARINE SYNECHOCOCCUS SP STRAINS
CELL-CYCLE REGULATION IN MARINE SYNECHOCOCCUS SP STRAINS
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DOI：
10.1128/aem.61.2.708-717.1995
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发表时间：
1995-02-01
影响因子：
4.4
通讯作者：
CHISHOLM, SW
中科院分区：
文献类型：
--
作者：
BINDER, BJ;CHISHOLM, SW
通过研究指数生长和暗封闭群体的DNA频率分布，并考虑在昼夜光暗周期下生长期间这些分布的变化模式，分析了四种海洋单细胞蓝细菌聚球藻菌株的细胞周期行为。这两种模式的细胞周期调控先前确定的淡水和沿海海洋聚球藻分离，分别代表了我们研究的三个开放的海洋菌株。第一种调节模式与广泛接受的原核细胞周期模式的缓慢生长情况一致。第二个似乎涉及染色体复制的异步启动，在低生长率下存在多个染色体拷贝，以及群体中细胞间染色体拷贝数的变异性。这些特征表明，参与细胞周期调控的概率大的组件，这可能会使应用程序的细胞周期为基础的估计原位生长速率聚球藻种群的问题。
The cell cycle behavior of four marine strains of the unicellular cyanobacterium Synechococcus sp. was analyzed by examining the DNA frequency distributions of exponentially growing and dark-blocked populations and by considering the patterns of change in these distributions during growth under a diel light-dark cycle. The two modes of cell cycle regulation previously identified in a freshwater and coastal marine Synechococcus isolate, respectively, were represented among the three open-ocean strains we examined. The first of these modes of regulation is consistent with the slow-growth case of the widely accepted prokaryotic cell cycle paradigm. The second appears to involve asynchronous initiation of chromosome replication, the presence of multiple chromosome copies at low growth rates, and variability in chromosome copy number among cells in the population. These characteristics suggest the involvement of a large probabilistic component in cell cycle regulation which could make the application of cell cycle-based estimators of in situ growth rate to Synechococcus populations problematic.