A transcription map of the minimally deleted region from 13q14 in B-cell chronic lymphocytic leukemia as defined by large scale sequencing of the 650 kb critical region.
A transcription map of the minimally deleted region from 13q14 in B-cell chronic lymphocytic leukemia as defined by large scale sequencing of the 650 kb critical region.
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B 细胞慢性淋巴细胞白血病 13q14 最小缺失区域的转录图谱，通过对 650 kb 关键区域的大规模测序确定。
DOI：
10.1038/sj.onc.1203978
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Cowell,JK
中科院分区：
文献类型：
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作者：
Kitamura,E;Su,G;Sossey-Alaoui,K;Malaj,E;Lewis,J;Pan,HQ;Hawthorn,L;Roe,B;Cowell,JK
对肿瘤的广泛分析表明，染色体13q14区域存在纯合子和杂合子缺失。3在B细胞慢性淋巴细胞白血病(B-CLL)中，提示肿瘤抑制基因的位置。由于以前对该基因的搜索没有发现任何可行的候选基因，我们现在给出跨越标记D13S319和D13S25之间最小缺失的约650kb区域的BAC序列。这一序列使我们能够为该区域创建最终的转录图谱，其中显示了该区域的93个EST和12个Unigene簇。利用基因预测程序，还识别了另外19个潜在基因。这些基因在整个地区表现出不对称的分布，其中大多数聚集在最末端。这种测序工作提供了B-CLL缺失区的最终结构，并鉴定了绝大多数潜在的候选基因。在所有已鉴定的基因中，只有三个与已知基因同源：两个L1重复基因和兔附睾蛋白52。这是13Q14。3序列提供了鉴定B-CLL抑癌基因的最终底物。
Extensive analysis of tumors has demonstrated homozygous and heterozygous deletions in chromosome region 13q14. 3 in B-cell chronic lymphocytic leukemia (B-CLL), suggesting the site of a tumor suppressor gene. Since previous searches for this gene have not yielded any viable candidates, we now present the sequence of the BACs which span the minimally deleted approximately 650 kb region between markers D13S319 and D13S25. This sequence has allowed us to create the definitive transcription map for the region which reveals 93 ESTs and 12 Unigene clusters in this region. Using gene prediction programs, a further 19 potential genes are also identified. The genes show an asymmetrical distribution throughout the region with most of them clustering at the extreme ends. This sequencing effort provides for the definitive structure of the B-CLL deletion region and the identification of the vast majority of the potential candidate genes. Of all the genes identified, only three have homologies to known genes: two L1 repeat genes and rabbit epididymal protein 52. This 13q14. 3 sequence provides the final substrate from which to characterize the B-CLL tumor suppressor gene.