Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum β-lactamases
Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum β-lactamases
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DOI：
10.1016/j.ijantimicag.2010.06.007
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发表时间：
2010-10-01
影响因子：
10.8
通讯作者：
Woodford, Neil
中科院分区：
文献类型：
--
作者：
Dhanji, Hiran;Doumith, Michel;Woodford, Neil
过去十年中，CTX-M-15 已成为许多国家大肠杆菌中最常见的广谱 β-内酰胺酶。它的优势部分反映了通常产生这种酶的大肠杆菌 O25b:H4 ST131 克隆的传播。我们描述了快速实时聚合酶链反应 (PCR) 测定，能够检测属于 ST131 克隆的大肠杆菌并识别 bla(CTX-M-15) 样。 (C) 2010 Elsevier B.V. 和国际化疗协会。版权所有。
CTX-M-15 has become the most prevalent extended-spectrum beta-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla(CTX-M-15)-like. (C) 2010 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.