Effect of selenium status on mRNA levels for glutathione peroxidase in rat liver.
Effect of selenium status on mRNA levels for glutathione peroxidase in rat liver.
复制标题
硒状态对大鼠肝脏谷胱甘肽过氧化物酶 mRNA 水平的影响。
DOI：
10.1016/s0006-291x(88)81174-4
复制
发表时间：
1988
影响因子：
3.1
通讯作者：
Sunde,RA
中科院分区：
文献类型：
--
作者：
Saedi,MS;Smith,CG;Frampton,J;Chambers,I;Harrison,PR;Sunde,RA
为了确定硒状态对硒依赖性谷胱甘肽过氧化物酶（EC 1.11.1.9）的mRNA水平的影响，给大鼠喂食缺硒酵母饮食（<0.02 mg Se/kg饮食）或硒充足饮食（+0.2 mg Se/kg Na 2SeO 3）>135 d。硒缺乏大鼠肝脏谷胱甘肽过氧化物酶活性为0.025，硒充足大鼠肝脏谷胱甘肽过氧化物酶活性为0.615 EU/mg蛋白。分离总肝RNA和聚腺苷酸化RNA，并使用来自克隆的鼠谷胱甘肽过氧化物酶的700 bp DNA探针进行北方印迹分析。放射自显影显示，硒缺乏的肝脏有7-17%的谷胱甘肽过氧化物酶的mRNA存在于硒充足的肝脏，这表明硒状态可能会调节这种硒酶的mRNA水平。
To determine the effect of Se status on the level of mRNA for Se-dependent glutathione peroxidase (EC 1.11.1.9), rats were fed either a Se-deficient torula yeast diet (<0.02 mg Se/kg diet) or a Se-adequate diet (+0.2 mg Se/kg as Na2SeO3) for >135 d. Liver glutathione peroxidase activity was 0.025 for Se-deficient versus 0.615 EU/mg protein for Se-adequate rats. Total liver RNA and polyadenylated RNA were isolated and subjected to Northern blot analysis using a 700 bp DNA probe from cloned murine glutathione peroxidase. Autoradiography showed that Se-deficient liver had 7–17% of the mRNA for glutathione peroxidase present in Se-adequate liver, suggesting that Se status may regulate the level of mRNA for this selenoenzyme.