New insights into the genomic structure of the oats (Avena L., Poaceae): intragenomic polymorphism of ITS1 sequences of rare endemic species Avena bruhnsiana Gruner and its relationship to other species with C-genomes
New insights into the genomic structure of the oats (Avena L., Poaceae): intragenomic polymorphism of ITS1 sequences of rare endemic species Avena bruhnsiana Gruner and its relationship to other species with C-genomes
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DOI：
10.1007/s10681-021-02956-z
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发表时间：
2022-01-01
期刊：
影响因子：
1.9
通讯作者：
Rodionov, A., V
中科院分区：
文献类型：
--
作者：
Gnutikov, A. A.;Nosov, N. N.;Rodionov, A., V
阿塞拜疆（Apsheron半岛）特有的二倍体C基因组燕麦Avena bruhnsiana的起源已被阐明。这种二倍体燕麦仅在阿塞拜疆的阿普舍龙半岛发现，与另一种二倍体燕麦A. ventricosa属于C基因组燕麦类群。在培养中，这些物种形成可育后代，但不与其他C基因组燕麦，A。clauda和A. pilosa。为了获得更精确的系统发育图，我们使用了下一代序列方法，该方法允许获得整个标记序列池（在我们的情况下，18 S rDNA（片段）-ITS 1 -5.8S rDNA）。基于NGS计数，我们发现至少67%的A。bruhnsiana rDNA来自A.脑室A. bruhnsiana可能是A.克劳达。clauda本身似乎是一个同倍体杂种：它的一个主要核糖体类型与A。而其中一个是分开的。此外，A. pilosa与A基因组有关（可能是祖先状态？）。多年生A. macrostachya，具有CmCm基因组，也是我们研究的对象。它在其他C基因组燕麦中具有两个密切相关的主要核糖体类型。大穗燕麦（Avena macrostachya ribotypes）仅与A. clauda A. pilosa复合体通过次要核糖体型组分。
The origin of diploid C-genome oat Avena bruhnsiana, which is a rare endemic species from Azerbaijan (Apsheron Peninsula) has been clarified. This diploid oat was found only in Apsheron Peninsula in Azerbaijan and is closely related to another diploid species, A. ventricosa that belongs to C-genome oat group. In culture these species form fertile progeny but do not cross with other C-genome oats, A. clauda and A. pilosa. For more precise phylogenetic picture we used the next-generation sequence method that allows to obtain the whole pool of marker sequences (in our case, 18S rDNA(fragment)-ITS1-5.8S rDNA). Based on NGS counts, we revealed that at least 67% of A. bruhnsiana rDNA were received from A. ventricosa. The second ancestor of A. bruhnsiana is probably A. clauda. Avena clauda itself appears to be a homoploid hybrid: one of its main ribotypes is identical to A. pilosa and one of them is separate. Also, one of the minor ribotypes of A. pilosa is related to A-genomes (probably, ancestral state?). The only tetraploid in the genus, perennial A. macrostachya, has CmCm-genome and was also studied in our work. It takes distant position among other C-genome oats having two closely related main ribotypes. Avena macrostachya ribotypes is connected only with A. clauda/A. pilosa complex via minor ribotype fraction.