Early morphometric and anisotropic change in periarticular cancellous bone in a model of experimental knee osteoarthritis quantified using microcomputed tomography
Early morphometric and anisotropic change in periarticular cancellous bone in a model of experimental knee osteoarthritis quantified using microcomputed tomography
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DOI：
10.1016/s0268-0033(00)00023-1
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发表时间：
2000-10-01
影响因子：
1.8
通讯作者：
Zernicke, RF
中科院分区：
文献类型：
--
作者：
Boyd, SK;Müller, R;Zernicke, RF
Objective.目的：建立实验性骨关节炎犬前交叉韧带横断模型，量化早期关节周围松质骨的显微结构变化。在10只动物中进行单侧前交叉韧带横断，在横断后3周和12周分别对5只动物的骨结构变化进行定量。另外两只未手术的动物用作对照。创伤后骨关节炎早期关节周围松质骨的小梁结构变化尚不清楚。先前的研究已经发现实验性骨关节炎中骨矿物质密度的改变，表明骨小梁结构的适应。韧带损伤后关节周围骨的早期改变可能有助于骨关节炎的长期发展。用三维显微断层扫描系统扫描股骨和胫骨对内侧髁的骨芯。测量骨体积比、骨表面积比、骨小梁厚度、骨小梁间距、骨小梁数量以及平均截距法测定的骨结构各向异性。在横断后3周观察到显著的结构变化，并且在横断后12周更加突出。这些变化伴随着各向异性降低。实验性骨关节炎关节周围松质骨显微结构发生显著改变。这些变化早在横断后3周就发生了，并且在横断后12周时较大。
Objective. To quantify early stage microstructural changes of periarticular cancellous bone in a canine anterior cruciate ligament transection model for experimental osteoarthritis.Design. Unilateral transection of the anterior cruciate ligament was performed in 10 animals, Bone structure changes were quantified in five animals at 3-week post-transection and five animals at 12-week post-transection. An additional two non-operated animals were used as controls.Background. Changes in trabecular architecture of the periarticular cancellous bone in early stage post-traumatic osteoarthritis is not well understood. Previous studies have found alterations in bone mineral density in experimental osteoarthritis suggesting adaptation of the trabecular structure. Early change of the periarticular bone following a ligament injury may contribute to the longterm development of osteoarthritis.Methods. Bone cores from the medial condyles of the femoral and tibial pairs were scanned with a three-dimensional microtomographic system. Structural indices were quantified including bone volume ratio, bone surface ratio, trabecular thickness, trabecular separation, trabecular number, as well as structural anisotropy determined by the mean-intercept-length method.Results. Significant structural changes were observed at 3-week post-transection, and were more prominent at 12-week post-transection. These changes were accompanied by decreasing anisotropy.Conclusions. Periarticular cancellous bone microstructure is significantly altered in experimental osteoarthritis. These changes occurred as early as 3-week post-transection, and were large at 12-week post-transection.