Express and portable label-free DNA detection and recognition with SERS platform based on functional Au grating
Express and portable label-free DNA detection and recognition with SERS platform based on functional Au grating
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DOI：
10.1016/j.apsusc.2018.11.092
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发表时间：
2019-03-15
影响因子：
6.7
通讯作者：
Lyutakov, Oleksiy
中科院分区：
文献类型：
--
作者：
Guselnikova, Olga;Postnikov, Pavel;Lyutakov, Oleksiy
基于无标记 SERS 的 DNA 检测被认为是便携式和快速生物分析中最具前景的分析方法之一。在这项工作中，基于功能性表面等离子体激元支持的有序结构的SERS平台被提出作为检测特定DNA序列的极其方便和有效的工具。设计的SERS结构使得使用便携式拉曼光谱仪进行近实时的DNA分析成为可能，结果具有高再现性和准确性，检测限达到10(-14) M量级。为了克服与拉曼信号的细致关联和评估相关的挑战，使用原始SERS数据的主成分分析(PCA)进行数据评估。当通过将寡核苷酸接枝到金表面及其与互补、错配和非互补寡核苷酸的进一步相互作用来实现分析功能时，通过创建具有均匀分布的等离激元能量的周期性结构来实现SERS结果的再现性。实验证明，利用 SERS 平台，不仅可以检测和识别纯 DNA，还可以同时检测和识别互补 DNA 和错配 DNA，并估计它们的相对浓度。此外，所开发的SERS传感器在3个月的存储期内表现出完美的稳定性，而没有丧失功能。
Label-free SERS-based detection of DNA is considered as one of the most prospective analytical methods for portable and express bioanalysis. In this work, the SERS platform based on the functional surface plasmon polariton supported ordered structure is proposed as an extremely convenient and effective tool for the detection of specific DNA sequences. The designed SERS structure makes possible DNA analyses in near-real time using the portable Raman spectrometer, with high reproducibility and accuracy of the results and the detection limits of the order of 10(-14) M. In order to overcome challenges, connected to meticulous affiliation and evaluation of Raman signals, principal component analysis (PCA) of raw SERS data was used for data evaluation. SERS results reproducibility was achieved through the creation of a periodical structure with homogenous distribution of plasmon energy, when the analysis functionality was implemented through the grafting of the oligonucleotide to gold surface and its further interaction with complementary, mispaired and noncomplementary oligonucleotides. It was demonstrated experimentally that with the SERS platform it is possible not only to detect and recognize pure DNA but also the simultaneous presence of complementary and mispaired DNA with estimation of their relative concentrations. Moreover, the developed SERS sensor demonstrated the perfect stability over 3 months storage period without loss of functionality.