Probing lysosomal activity in vivo.
Probing lysosomal activity in vivo.
复制标题
探测体内溶酶体活性
DOI：
10.52601/bpr.2021.200047
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发表时间：
2021-02-28
影响因子：
--
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Li X;Sun Y;Wang X
溶酶体是用于生物分子降解和再循环的膜结合细胞器。它们还作为营养传感和信号中心来维持细胞和组织的稳态。溶酶体的性质会随着发育或环境的变化而改变，但这些变化在体内很难追踪。使用C。作为模型系统，我们已经开发了检测和定量体内溶酶体性质的测定法，包括溶酶体成熟、酸化和切割活性。这些试验可用于揭示C. elegans的发展和压力条件。
Lysosomes are membrane-bound organelles for biomolecule degradation and recycling. They also serve as a nutrient sensing and signaling center to maintain cell and tissue homeostasis. Lysosomal properties alter in response to developmental or environmental cues, but these changes are hard to track in vivo. Employing C. elegans as a model system, we have developed assays to examine and quantify lysosome properties in vivo, including lysosome maturation, acidification and cleavage activity. These assays can be used to reveal alterations of lysosomal activity during C. elegans development and in stress conditions.