Using siRNA technique to generate transgenic animals with spatiotemporal and conditional gene knockdown
Using siRNA technique to generate transgenic animals with spatiotemporal and conditional gene knockdown
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DOI：
10.1016/s0002-9440(10)63411-6
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发表时间：
2004-11-01
影响因子：
6
通讯作者：
Yu, WCY
中科院分区：
文献类型：
--
作者：
Chang, HS;Lin, CH;Yu, WCY
Based on the RNAi technique, we have developed a new approach that generates transgenic animals capable of mimicking human genetic diseases. The new system is a combination of siRNA with Cre-loxP and tetracycline-on. it has the characteristics of being stable, inheritable, and inducible, with the siRNA able to be transcribed tissue specifically. To support the ability of this new method to generate a model for a disease, we created an ABCA1-deficient mouse line that mimics Tangier disease under controlled conditions. Thus, it should now be possible to rapidly establish human genetic diseases as a whole animal model without the use of embryonic stem cell and gene targeting. This system also provides a tool for pathological and pharmacological studies of aspects peculiar to particular human genetic diseases.
