Serotonin Activates Catecholamine Neurons in the Solitary Tract Nucleus by Increasing Spontaneous Glutamate Inputs
Serotonin Activates Catecholamine Neurons in the Solitary Tract Nucleus by Increasing Spontaneous Glutamate Inputs
复制标题
血清素通过增加自发谷氨酸输入来激活孤束核中的儿茶酚胺神经元
DOI：
10.1523/jneurosci.1372-12.2012
复制
发表时间：
2012-11-14
影响因子：
5.3
通讯作者：
Appleyard, Suzanne M.
中科院分区：
文献类型：
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作者：
Cui, Ran Ji;Roberts, Brandon L.;Appleyard, Suzanne M.
5-羟色胺（5-HT）是控制自主神经功能的关键神经递质。5-HT 3受体参与迷走神经传入反馈以减少食物摄入并调节心血管反射;然而，涉及的孤束核（NTS）神经元的表型尚不清楚。NTS中的A（2）/C-2儿茶酚胺（CA）神经元直接被内脏传入激活，并且对于控制食物摄入和心血管功能是重要的，使它们成为在NTS水平响应和介导5-羟色胺效应的良好候选者。本研究采用膜片钳技术检测5-羟色胺对NTS-CA神经元的影响，并采用酪氨酸羟化酶（TH）启动子驱动的增强型绿色荧光蛋白（TH-EGFP）转基因小鼠来鉴定儿茶酚胺神经元。5-羟色胺增加了>90%的NTS-TH-EGFP神经元中自发性谷氨酸兴奋性突触后电流（sEPSC）的频率，该作用被5-HT 3受体拮抗剂昂丹司琼阻断，并被5-HT 3受体激动剂SR 5227和mCPBG模拟。相比之下，5-HT 3受体激动剂增加了少数（
Serotonin (5-HT) is a critical neurotransmitter in the control of autonomic functions. 5-HT3 receptors participate in vagal afferent feedback to decrease food intake and regulate cardiovascular reflexes; however, the phenotype of the solitary tract nucleus (NTS) neurons involved is not known. A(2)/C-2 catecholamine (CA) neurons in the NTS are directly activated by visceral afferents and are important for the control of food intake and cardiovascular function, making them good candidates to respond to and mediate the effects of serotonin at the level of the NTS. This study examines serotonin's effects on NTS-CA neurons using patch-clamp techniques and transgenic mice expressing an enhanced green fluorescent protein driven by the tyrosine hydroxylase (TH) promoter (TH-EGFP) to identify catecholamine neurons. Serotonin increased the frequency of spontaneous glutamate excitatory postsynaptic currents (sEPSCs) in >90% of NTS-TH-EGFP neurons, an effect blocked by the 5-HT3 receptor antagonist ondansetron and mimicked by the 5-HT3 receptor agonists SR5227 and mCPBG. In contrast, 5-HT3 receptor agonists increased sEPSCs on a minority (