The novel primers for sex identification in the brown eared‐pheasant and their application to other species

The novel primers for sex identification in the brown eared‐pheasant and their application to other species
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DOI:
10.1111/j.1755-0998.2008.02177.x
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发表时间:
2009-01
影响因子:
7.7
通讯作者:
N. Wang;Z. Zhang
N. Wang;Z. Zhang
中科院分区:
生物学1区
文献类型:
--
作者:
N. Wang;Z. Zhang

文献摘要

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根据PCR扩增CHD片段的原理,设计了一对用于褐马鸡性别鉴定的引物,并对扩增产物的数量进行了鉴定。新的引物比P2/P8具有更高的灵敏度,跨种应用表明,它们也可用于雀科和雀形目其他物种的性别鉴定。
We designed a pair of primers for sex identification in the brown eared‐pheasant (Crossoptilon mantchuricum) based on the mechanism of PCR amplification of CHD fragments, and identified the number of products. The new primers were considered to have more sensitivity than P2/P8, and cross‐species application indicated that they can also be used for sex identification in other species of Phasianidae and Passeriformes.